急性心理应激对小鼠记忆的影响

Effect of acute psychological stress model on learning and memory in mice

目的 研究急性心理应激对小鼠学习记忆功能的影响。方法 雄性昆明小鼠42只随机分成4组,即:八臂迷宫心理应激组(A)、八臂迷宫对照组(B)、Barnes迷宫心理应激组(C)、Barnes迷宫对照组(D),每组10只。旁观应激2d后,用放射状八臂迷宫和Barnes迷宫进行空间学习记忆能力的测试。结果 1.心理应激后小鼠完成八臂迷宫的时间[(527.4±95.15)s]较应激前[(362.20±70.61)s]延长,错误的臂数(6.70±3.34)较应激前增加,首错前连续正确的臂数(4.0±1.83)较应激前减少(P<0.01),与对照组比较也出现同样结果。2.心理应激后小鼠完成:Barnes迷宫时间[(111.1±67.43)s]较应激前时间[(59.92±32.81)s]延长,错误的洞数(9.6±6.31)较应激前增加(P<0.01),对照组的比较也得到同样的结果。结论急性心理应激可以损伤小鼠的空间学习和工作记忆能力。

Objective To study the effect of acute psychological stress model on learning and memory in mice. Methods Forty-two Kun-ming mice were divided into four groups : 8-arm radial maze-stressed group ( A) . 8-arm radial maze of non-stressed group (B) , Barnes maze of stressed group(C) , Barnes maze of non-stressed group(D). The changes of learning and memory behavior of mice were respectively observed with 8-arm radial maze and Barnes maze after 2 day psychological stress. Results 1. 8-arm radial maze-stressed group (A) significantly more time in completing 8-arm maze than 8- arm radial maze of non-stressed group (B) and wrong number of arm are significantly increase also ( P<0. 05) .2. Barnes maze of stressed group(C) compared with Barnes maze of non-stressed group (D) more time were used in completing maze and wrong number of hole are significantly increase also ( P<0. 05). Conclusions These observations suggest the acute psychological stress model can result in the mice learn and remember failing.