缺氧缺血肺损伤新生鼠肺组织细胞凋亡及Fas/FasL mRNA的表达和意义

The changes in apoptosis and Fas/FasL expression in newborn rats with lung injury by hypoxia

目的:探讨细胞凋亡与缺氧缺血肺损伤发生、发展的关系以及Fas/FasL系统表达的意义。方法:通过复制缺氧、缺氧后吸入纯氧大鼠肺损伤模型,采用TUNEL法、原位杂交检测、SqRT-PCR技术观察肺组织细胞凋亡情况、FasmRNA、FasLmRNA表达强度。结果:缺氧组或缺氧吸入纯氧组在缺氧4h后即可见大鼠肺泡上皮细胞和肺血管内皮细胞出现凋亡现象,并随着时间延长,细胞凋亡指数增高(P=0.003,P=0.005),同时FasmRNA、FasLmRNA表达上调,且缺氧后吸入纯氧组大鼠FasmRNA、FasLmRNA表达较单纯缺氧组明显增强(P<0.05)。结论:细胞凋亡与FasmRNA、FasLmRNA表达的上调可能参与缺氧和缺氧后吸入纯氧时导致的肺损伤。

Objectives To observe the expression of apoptosis related gene Fas/FasL on lung injury caused by hypoxia and inhaled 100%O2 after hypoxia. Methods Two lung injury rat models were established by hypoxia or inhaled 100%O2 respectively. Apoptosis of the lung tissue was observed with the methods of Tunel and in situ hybridization. mRNA expression of Fas/FasL was detected by SqRt PCR. Results Apoptosis of rat pulmonary alveolar epithelial cells and pneumoangiogram endothelial cells appeared after 4 hours in two groups. And the index of apoptosis increased with the time passing by (P=0.003, 0.005 respectively). And expression of Fas/FasL mRNA or proteins was up regulated in two groups, which in inhaled pure oxygen group was higher than in hypoxia group. Conclusions Apoptosis and upregulated Fas/FasL mRNA and its relatedproteins play an important role on lung injury which was caused by hypoxia or inhaled pure oxygen after hypoxia.