米诺环素对人牙周韧带细胞矿化能力的影响

The effect of minocycline on the mineral characteristics of human periodontal ligament cells

目的探讨米诺环素对人牙周韧带细胞(periodontal Iigament cells,PDLCs)碱性磷酸酶(alkaline phosphatase,ALP)活性及矿化结节形成能力的影响。方法将不同浓度的米诺环素(0,1,5,20,100,200mg/L)加入体外培养的第4代HPDLcs中,孵育4天后,检测其对细胞ALP活性的影响;将第4代的PDLCs在体外进行长期培养,实验组加入矿化液和米诺环素(20mg/L或100mg/L),对照组只加矿化液。28天后用茜素红与VonKossa染色检测矿化结节的形成。结果20mg/L和100mg/L的米诺环素能显提高PDLCsS的ALP活性,其中20mg/L具有最大的促进效应,对体外矿化结节的形成,亦有一定程度的促进作用。结论米诺环素有促进牙周韧带细胞向成骨细胞方向转化的趋势,从而有助于牙周新附着的形成。

Objective To investigate the effect of minocycline on the alkaline phosphatase (ALP) activity and mineralized nodules formation of human periodontal ligament cells ( PDLCs) . Methods Various concentrations of minocycline (0, 1, 5, 20, 100, 200mg/L) were added to the medium of cultured fourth passage HPDLCs. After 4 days of co-incubation, the ALP activity was measured. The fourth passage PDLCs was cultured for 4 weeks, mineralized solution and minocycline (20mg/L or 100mg/L) were added into the test group medium. Mineralized solution was added into the medium of the control group. At day 28, the formation of mineralized nodules was detected with Dahl McGec-Russll's alizarin red stain and von Kossa's silver nitrate staining for calcium. Results Minocycline at concentrations of 20mg/L and 100mg/L could increase ALP activity; the 20mg/L minocycline had an optimal effect (P <0.01). Minocycline could also induce nodule formation to some degree. Conclusion Minocycline has the tendency of inducing PDLCs to differentiate into osteoblastic cells. Therefore, it may be helpful for the formation of periodontal new attachment.