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固醇调节元件结合蛋白-1c(SREBP-1c)对小鼠Ⅰ型葡萄糖载体(mGLUT1)的表达调节 被引量：1

Sterol regulatory element binding protein-1c(SREBP-1c) activates mouse Glucose transporter I(mGLUT1) expression
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摘要 目的探讨SREBP1c对mGLUT1表达的影响。方法采用分子克隆技术,将SREBP1c和mGLUT1cDNA分别克隆到表达载体pSVsport和pGL3b上。SREBP1c与mGLUT1克隆载体同时或单独转染NIH3T3细胞,然后应用荧光素酶活性测定法、RTPCR及Northernblot等方法测定SREBP1c对mGLUT1的启动子活性调节及mRNA表达的影响。结果实验结果表明,SREBP1c可激活mGLUT1启动子的活性,并且可增加内源性mGLUT1的mRNA表达水平。结论SREBP1c可增强mGLUT1的表达,可能介导胰岛素对mGLUT1的调节过程。 Objective To understand the possibility of contribution of SREBP-1c for mGLUT1 expression.Methods SREBP-1c and mGLUT1 cDNA clone into pSV-sport, pGL3b expression vector. The recombinant SREBP-1c and mGLUT1 transfected into NIH 3T3 cells, and confirm the mGLUT1 promoter activity and mRNA expression by the SREBP-1c with luciferase assay, RT-PCR and Northern blot.Results SREBP-1c activates the mGLUT1 promoter activity and increase the mGLUT1 mRNA expression. Conclusion SREBP-1c activates mGLUT1 expression, and may meditate mGLUT1 expression by insulin.
作者 李天洙 张学武 柳明洙 崔长旭 孙权 陈丽艳 尹宗柱
机构地区 延边大学医学院生物化学与分子生物学教研室
出处 《中国实验诊断学》 2004年第6期575-577,共3页 Chinese Journal of Laboratory Diagnosis
基金 BK(Brain korea)21科研项目
关键词 固醇调节元件结合蛋白-1c(SREBP-1c) 小鼠Ⅰ型葡萄糖载体(mGLUTI) NORTHERN BLOT MRNA表达 SREBP-1c mGLUT1 Northern blot mRNA expression
分类号 Q756 [生物学—分子生物学]
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参考文献11

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同被引文献14

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中国实验诊断学
2004年 第6期

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