金喜素对原代培养的人食管癌细胞的抗癌作用

Anti-tumor effects of Topotecan on primary cultured human esophagus cancer cells

目的:研究金喜素(Topotecan)对体外原代培养的人 食管癌细胞增殖与凋亡作用的影响及可能的机制.方法:原 代培养人食管癌细胞,MTT法检测金喜素对食管癌细胞增殖 作用的影响;吖啶橙染色观察凋亡细胞形态学变化;流式细胞 仪定量检测金喜素处理细胞后细胞凋亡情况;底物裂解法检 测金喜素对食管癌细胞半胱天冬酶 3(caspase 3)活性的影 响.结果:金喜素处理细胞48h后,可明显抑制癌细胞增殖, 并呈浓度依赖性;金喜素处理的食管癌细胞经吖啶橙染色可 检测到大量凋亡细胞,流式细胞仪检测其凋亡率为(33.4± 6.8)%,而未经金喜素处理组几乎未检测到凋亡细胞;金喜素 处理食管癌细胞后其Caspase 3活性较未处理组显著增高. 结论:金喜素可抑制体外原代培养的食管癌细胞增殖,并通 过促进Csapase 3活性而诱导细胞凋亡.

AIM: To study the effect of Topotecan on the proliferation and apoptosis of primary cultured esophagus cancer cells and its possible mechanism. METHODS: Primary cultured esophagus cells were treated with topotecan of different concentrations for 48 h. MTT assay was used to detect the viability of cells. Morphological changes of apoptotic cells were studied by acridine orange staining and the apoptotic rates were detected by flow cytometer. Caspase-3 activity was measured by colorimetric assay. RESULTS: After treatment with topotecan for 48 h, the cell proliferation was significantly inhibited in a dose-dependent manner. Typical morphological changes of apoptotic cells could be detected by acridine orange staining in cells treated with topotecan with an apoptotic rate of (33.4±6.8)% but few apoptotic cells could be detected in cells without topotecan incubation. Topotecan markedly increased the caspase-3 activity of esophagus cancer cells after treatment for 48 h. CONCLUSION: Topotecan can inhibit the proliferation of primary cultured esophagus cancer cells and induce apoptosis through the activation of caspase-3.