当归多糖对骨髓巨噬细胞的影响及其与造血调控的关系

Effect of angelica polysaccharide on bone marrow macrophage and its relationship to hematopoietic regulation

目的研究当归多糖(APS)对骨髓巨噬细胞(BMMΦ)生物活性的影响及其与造血调控的关系,从而阐明当归的活血机制。方法采用骨髓造血祖细胞和巨噬细胞体外培养,造血生长因子生物活性检测,免疫细胞化学,核酸探针原位杂交等技术。结果经APS诱导的BMMΦ的培养上清液可提高髓系造血祖细胞的集落产率;经APS诱导后,BMMΦ表达促红细胞生成素(EPO)、粒-巨噬细胞集落刺激因子(GM-CSF)、白细胞介素-3(IL-3)、白细胞介素-6(IL-6)的蛋白水平有不同程度的提高;EPOmRNA和GM-CSFmRNA的表达水平和强度明显提高。结论APS可以直接和(或)间接地刺激造血诱导微环境中的BMMΦ,从基因水平和蛋白水平上促进造血调控因子的合成和分泌,进而促进髓系多向性造血祖细胞(CFU-Mix)、晚期红系祖细胞(CFU-E)、粒单系造血祖细胞(CFU-GM)的增殖分化。

Objective To investigate the effect of angelica polysaccharide (APS) on bioactivity of bone marrow macrophage (BMMΦ) and its relationship to hematopoietic regulation, for clarifying the hematonic mechanism of Angelica sinensis. Methods The techniques of hematopoietic progenitor cell culture and BMMΦ culture in vitro, biological assay of hematopoietic growth factor (HGF) in culture media of BMMΦ, immunocytochemistry, and nucleic acid in situ hybridization were used. Results The culture supernatant of BMMΦ induced by APS can enhance the CFU-Mix, CFU-E, CFU-GM; the expression of erythropoietin (EPO), GM-CSF, IL-3, IL-6 protein in BMMΦ induced by APS was much stronger than that in the control group at different levels; the expression of EPO mRNA and GM-CSF mRNA in BMMΦ induced by APS were intensified. Conclusion APS may directly and/or indirectly stimulate the BMMΦ in hematopoietic inductive microenvironment to accelerate the synthesis and secretion of hematopoietic regulation factors on the basis of gene and protein level, such as EPO, GM-CSF, IL-3, IL-6, which in turn to promote the proliferation and differentiation of CFU-Mix, CFU-E, and CFU-GM.