摘要
目的 探讨流式细胞免疫分型在抗原异常表达淋巴瘤诊断及鉴别诊断中的作用。方法 应用流式细胞术对伴有抗原异常表达的淋巴瘤 3例 ,B淋巴细胞性白血病 8例 ,T淋巴细胞性白血病 1例及伴有淋巴细胞分化抗原表达的急性非淋巴细胞白血病 17例 ,急性粒细胞白血病累及淋巴结1例的免疫表型进行回顾性分析 ,每例均作胞质CD3ε、CD79a、髓过氧化物酶 (MPO)的EnVision两步法染色。结果 11例B细胞淋巴瘤 /白血病胞质CD79a阳性 ,胞质CD3ε及MPO阴性 ,其中 5例伴CD5表达 ,2例同时伴CD5及 1～ 2项髓系分化抗原表达 ;1例T淋巴细胞白血病胞质CD3ε阳性 ,胞质CD79a及MPO阴性 ,伴CD13、CD33表达 ;18例急性非淋巴细胞白血病 (包括 1例急性粒细胞白血病累及淋巴结 )胞质髓过氧化物酶 (MPO)均阳性 ,胞质CD3ε及CD79a均阴性 ,其中 8例 (包括 1例急性粒细胞白血病累及淋巴结 )伴T细胞分化抗原表达 ,8例伴B细胞分化抗原表达 ,2例同时伴T细胞及B细胞分化抗原表达。结论 流式细胞免疫分型不仅能发现淋巴瘤 /白血病抗原异常的表达 ,而且可通过表面免疫标记及胞质CD3ε、CD79a、MPO的表达来确定抗原异常表达淋巴瘤 /白血病的细胞来源 ,对抗原异常表达淋巴瘤 /白血病的诊断及鉴别诊断具有十分重要价值。
Objective To investigate the application of flow cytometry in the differential diagnosis of lymphoma / leukemia with aberrant antigen expression Methods The results of flow cytometry of 30 lymphoma / leukemia cases with aberrant antigen expression, of which 3 cases being lymphomas, 8 B cell leukemia, 1 T cell leukemia, 17 acute non lymphoid leukemia and 1 acute non lymphoid leukemia involving lymph nodes were analyzed Immunohistochemistry (EnVision) for CD79a, CD3 and MPO was performed on all cases Results Eleven cases of B cell lymphoma / leukemia were cytoplasmic CD79a (cCD79a) positive, cytoplasmic CD3 (cCD3ε) and cytoplasmic MPO (cMPO) negative Five of these cases were positive for CD5 and 2 for CD5, 1 or 2 for myeloid marker(s) The T cell leukemia cases were cCD3ε positive, cCD79a and cMPO negative, they also co expressed CD13 and CD33 The mantle cell lymphoma cases were positive for CD3,CD13 and CD33 Of the 8 B cell leukemia cases, 4 were positive for CD5, 3 for CD13 and 1 for CD13 and CD33 The 18 acute non lymphoid leukemia cases (including 1 acute non lymphoid leukemia case involving lymph nodes) were cMPO positive and cCD79a and cCD3ε negative Eight of the 18 expressed T cell markers (including 1 case of acute non lymphoid leukemia involving lymph nodes), 8 expressed B cell markers, 2 expressed both T and B cell markers Conclusions Flow cytometry can demonstrate aberrant antigen expression in lymphoma /leukemia cells and is helpful in delineating their cell origin The technique is thus useful in the differential diagnosis of lymphoma / leukemia
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2004年第6期532-535,共4页
Chinese Journal of Pathology
