大剂量HBsAg疫苗对HBV转基因小鼠细胞免疫调节的研究

Study on cellular immune response to large-dose HBsAg vaccine in transgenic mice

目的 研究大剂量HBsAg疫苗对HBV转基因小鼠 (Tg鼠 )细胞免疫应答的影响。方法 Tg鼠 4 8只 ,每只注射 6 μg(相当于人产生抗体剂量的 180倍 )的HBsAg疫苗后 ,用流式细胞术、氚 胸腺嘧啶核苷 (3 H TdR)掺入法和ELISA法 ,分别检测其树突状细胞(DC)、T淋巴细胞增殖及其诱生细胞因子IL 2、IFN γ的水平。结果 大剂量HBsAg疫苗组DC表面共刺激分子CD80、CD86和I Ek的阳性百分率 (% )均高于对照组 (P <0 0 5 ) ,特异T淋巴细胞增殖能力 8个月的cpm值 (10 0 77 2± 85 74 0 )显著高于 1周的 (5 32 9 1± 30 86 0 ) (P <0 0 5 ) ;其诱生细胞因子的水平 8个月时IL 2 (46 2 4± 12 2 5pg/ml)、IFN γ(976 1± 5 4 4 1pg/ml)显著高于 1周时IL 2 (15 6 1±78 7)、IFN γ(5 8 3± 4 9 5 ) (P <0 0 1)。结论 大剂量HBsAg疫苗能够增强Tg鼠细胞免疫应答能力 ,恢复对乙型肝炎抗原的应答。

Objective To study the cellular immune response to a large-dose of HBsAg vaccine in transgenic (Tg) mice. Method After the Tg mice was immunized with HBsAg vaccine, the number of dentritic cells (DC), the level of proliferation of T-lymphocytes, and the production of IL-2 and IFN-γwere determined. Results The percentage of cells positive for common stimulating factors CD80, CD86, and I-E k were significantly higher than that of the control group (P<0.01), the proliferation rate of specific T-lymphocyte after 8 months (10 077.2±8 574.0) was significantly higher than that in the first week(5 329.1±3 086.0)(P<0.01), and the production of cytokines was significantly higher than those after the first week (P<0.01). Conclusion A large dose of HBsAg vaccine can increase the cellular immune response in Tg mice and recover immune response to HBsAg.