摘要
目的 :研究蒺藜总皂苷 (GSTT)对大鼠急性心肌缺血损伤的保护作用。方法 :结扎大鼠左冠状动脉前降支建立急性心肌缺血模型 ,观察 GSTT对心肌缺血大鼠血清 L DH、 SOD和 MDA的影响 ;通过 HE染色、电镜观察心肌细胞形态学的变化 ;采用 TUNEL方法半定量观察 GSTT对凋亡细胞阳性率的影响。结果 :与对照组比较 ,GSTT可降低心肌缺血大鼠血清 L DH、 MDA (P<0 .0 1) ,升高 SOD (P<0 .0 1) ,降低心肌凋亡细胞阳性率 (P<0 .0 1) ;模型组大鼠心肌细胞结构出现明显的病理改变。结论 :GSTT对结扎大鼠左冠状动脉前降支引起的急性心肌缺血损伤具有保护作用 ,保护作用通过升高 SOD,清除自由基的能力增强及 L DH的释出减少进而抑制心肌细胞凋亡。
Objective To study the protective effects of Gross Saponins of Tribulus Terrestris (GSTT) on acute myocardial ischemia in rats. Methods An acute myocardial ischemic model was set up by ligating left coronary artery, the effects of GSTT on the serum LDH, SOD, and MDA contents in rats were observed. Meanwhile, HE staining and electron microscope observation were used to study the morphological changes of cardiomyocytes. The TUNEL semi-quantitative technique was performed to observe the effects of GSTT on apoptosis rate. Results Compared with control group, GSTT decreased the serum LDH and MDA contents(P<0.01),while it increased the serum SOD level (P<0.01) in rats. It decreased myocardial apoptosis rate as well(P<0.01). The obvious pathological changes of cardiomyocyte structure were found in model group.Conclusion GSTT has protective effects on acute myocardial ischemia induced by ligating left coronary artery in rats in vivo, which is closely related to enhancing removement of free radicals by increasing SOD content, decreasing the leakage of LDH, and inhibiting the apoptosis of cardiomyocytes
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2005年第1期1-4,F002,共5页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题 (30 371739
30 4 72 0 2 0 )
关键词
蒺藜/药理学
疾病模型
动物
心肌缺血
细胞凋亡/药物作用
蒺藜皂苷
Tribulus Terrestris/pharmacology
disease model,animal
myocardial ishemia
apoptosis/drug effects
Gross Saponins of Tribulus Terrestris
