乳腺癌原发灶和淋巴结转移灶内间质血管的比较

Comparison of microvessels between primary foci and lymph node metastasis in ductal breast carcinoma

目的 :对比研究乳腺癌原发灶和淋巴结转移灶内微血管密度及 VEGF、CD4 4 V6 、 CD34的表达规律 ,探讨肿瘤区域淋巴结内转移的机制及机体的相关反应。方法 :应用免疫组织化学超敏 SP法检测 30例伴有区域淋巴结转移乳腺癌的原发灶与转移灶及 8例无区域淋巴结转移乳腺癌 CD34、VEGF和 CD4 4 V6 的表达。结果 :原发灶内微血管密度明显高于转移灶 (P<0 .0 5 ) ;VEGF的表达两者间差异无显著性 ;原发灶内微血管密度和 VEGF表达呈正相关 (r=0 .396 3,P<0 .0 5 ) ,但转移灶内两者无明显相关性 (r=0 .0 94 ,P>0 .0 5 ) ;原发灶和转移灶间CD4 4 V6 表达差异无显著性 ;淋巴结转移阳性乳腺癌其微血管密度、VEGF、CD4 4 V6 的表达均明显高于淋巴结转移阴性者 (P<0 .0 5 )。结论 :乳腺癌的原发灶与淋巴结转移灶间血管生成能力不同 ,体内某些因素对淋巴结转移灶内的血管生成有抑制作用。

Objective To compare the microvessel density (MVD) and the expressions of vascular endothelial growth factor (VEGF), CD44V 6 and CD34 in ductal breast carcinoma between primary tumor and lymph node metastasis for researching the mechanism of metastasis and the corresponding reaction of body. Methods The expressions of CD34, VEGF, and CD44V 6 were detected and analyzed in 30 cases of breast cancer with lymph node involvement (LN +) and 8 cases of breast cancer without lymph node involvement (LN -) by immunohistochemical technique. Results Higher MVD was observed in the primary lesions than in the metastatic foci (P<0.05), and the expressions of VEGF were not significantly different between these two positions. There was a positive correlation between the expressions of VEGF and MVD in the primary tumor, and there was not a correlation between the expressions of VEGF and MVD in the metastatic foci in the lymph nodes. The expressions of CD44V 6 were not significantly different between primary lesions and metastatic foci. The expressions of VEGF, CD34 and CD44V 6 in the LN + primary lesions were higher than those in the LN - lesions (P<0.05). Conclusion The ability of angiogenesis between primary lesions of ductal breast carcinoma and its corresponding metastatic foci in the lymph nodes are different. Some elements in the metastatic foci can suppress the ability of angiogenesis.