胰腺癌p16、Bcl-2、Bax和VEGF基因表达及新生血管密度计数的意义

Expressions of p16, Bcl-2, Bax, and VEGF and significance of microvessel density in human pancreatic cancer

目的 :研究胰腺癌组织中 p16、 Bcl- 2、 Bax和 VEGF基因的表达及新生血管计数的意义。方法 :采用免疫组织化学法及形态半定量方法对 33例胰腺癌和 6例正常胰腺组织进行新生血管密度记数和 p16、Bcl- 2、Bax和 VEGF基因表达检测。结果 :P16蛋白表达 :高、中分化胰腺癌高于低分化 (P<0 .0 5 ) ,生存期长者表达高于生存期短者 (P<0 .0 5 ) ;Bcl- 2表达 :高、中分化者高于低分化者 ;Bax表达 :正常胰腺组织高于胰腺瘤组织 ,高、中分化者高于低分化者 ,生存期≥ 12个月者高于 <12个月者 ;VEGF表达 :有淋巴结转移者高于无淋巴结转移者 ;MVD记数 :正常胰腺组织低于胰腺瘤组织 ,有淋巴转移者高于无淋巴结转移者 ;MVD与 VEGF表达呈正相关(r=0 .79,P<0 .0 5 ) ;p16基因表达与 VEGF表达和 MVD呈负相关 (r=- 0 .6 9,P<0 .0 5 ;r=- 0 .87,P<0 .0 5 ) ,Bcl- 2表达与其他基因表达结果无相关关系。 Bax表达与 p16表达呈正相关 (r=0 .5 4 ,P<0 .0 5 ) ,与 VEGF表达和 MVD呈负相关 (r=- 0 .6 6 ,P<0 .0 5 )。结论 :综合检测胰腺癌组织中 p16、Bcl- 2、Bax和 VEGF基因的表达及微血管密度对判断胰腺癌恶性程度及估计肿瘤的预后有较大帮助。

Objective To study the expressions of p16, Bcl-2, Bax, VEGF and significance of microvessel density(MVD) in human pancreatic cancer. Methods Immunohistochemistry method and morphological semi-quantitative analysis were used to detect the quantity of MVD and the expressions of p16,Bcl-2,Bax, and VEGF in 33 cases of pancreatic cancer and 6 normal pancreas tissue specimen. Results The expression of P16 protein: it was higher in moderately or well differentiated pancreatic adenocarcinoma than poorly one (P<0.05), it was higher in patient with long survial time than those with short survial time (P<0.05);the expression of Bcl-2: it was higher in moderately or well differentiated pancreatic adenocarcinoma than poorly one; the expression of Bax:it was higher in normal pancreatic tissue than pancreatic cancer, as well as moderately or well differentiated than poorly one and survival time≥12 months than <12 months; the expression of VEGF:it was higher in patients with lymph node metastasis than without lymph node metastasis; MVD count: it was lower in normal pancreatic tissue than pancreatic cancer as well as with lymph node metastasis than without lymph node metastasis; the expression of MVD had positive correlation with VEGF expression (r=0.79,P<0.05);p16 gene expression had negative correlation with VEGF expression and MVD (r=-0.69,P<0.05;r=-0.87,P<0.05),but Bcl-2expression had no correlation with other gene expressions. Bax expression had positive correlation with p16 gene expresion (r=0.54,P<0.05),and had negative correlation with VEGF expression and MVD(r=-0.66, P<0.05). Conclusion The determination of p16, Bcl-2, Bax, VEGF expressions and MVD in human pancreatic cancer may be used to evaluate the prognosis and judge the differentiation of the adenocarcinoma.