TP53基因多态性与非小细胞肺癌易感性及辐射敏感性的关系

Association of polymorphism in TP53 gene with susceptibility and radiation sensitivity of non-small-cell-lung cancer in Chinese population

目的 :研究中国北方人非小细胞肺癌 (NSCL C)的易感性和放疗敏感性与 TP5 3基因多态性的关系。方法 :采用病例对照研究方法 ,通过合成序列特异性引物 (PCR- RFL P)方法检测 88例 NSCL C患者及 112例健康对照者的 TP5 3基因的基因型 ,观察不同基因型患者的放射治疗效果 ,筛检与辐射敏感性相关的基因型。结果 :NSCL C组的 C/ C等位基因频率明显高于对照组 (χ2 =5 .6 5 ,P=0 .0 17) ,NSCL C组的 C/ C基因型频率明显高于对照组 (χ2 =9.33,P=0 .0 0 2 3) ,C/ C纯合子患非小细胞肺癌的相对危险度为 2 .4 3(1.32～ 4 .5 1) ,G/ G纯合子患肺癌的相对危险度为 0 .90 (0 .4 6～ 1.75 )。 TP5 3基因多态性与非小细胞肺癌患者的辐射敏感性无关联。结论 :TP5 3基因的 C/ C基因型为非小细胞肺癌的易感基因 ,但尚未发现该基因型与 NSCL C的辐射敏感性相关。

Objective To study the association of polymorphism in TP53 gene with the susceptibility and radiation sensitivity of non-small-cell-lung cancer (NSCLC) of the population in the North of China. Methods Using RFLP-PCR assays, TP53 genotypes were detected by amplifying DNA fragments with sequence specific primers and digested by FnuDⅡ enzyme in 88 patients with NSCLC as well as 112 healthy controls. Results The C/C allele frequency was significantly higher in NSCLC patients than that in the healthy controls (χ 2=5.65,P=0.017) . The C/C genotype frequency was significantly higher in NSCLC patients than that of the healthy controls (χ 2=9.33, P=0.0023). The risk of C/C homozygotes in NSCLC patients was about 2.7 times against G/G homozygotes with odds ratio of 2.43 (95% CI=1.32-4.51). Conclusion In the population in the North of China, TP53 C/C genotype is closely associated with the susceptibility of NSCLC. There is no significant relationship between the polymorphism in TP53 gene and radiation sensitivity in NSCLC.