CTLA4-Ig对支气管哮喘患者外周血IL-13的影响

Effects of CTLA4-Ig on IL-13 in peripheral blood of patients with bronchial asthma

目的 :通过对支气管哮喘患者外周血 T细胞表达 IL- 13的情况及 CTL A4 - Ig对其影响的研究 ,进一步明确支气管哮喘的免疫发病机制并为治疗支气管哮喘寻求一条安全有效的途径。方法 :用 EL ISA和逆转录 PCR方法测定支气管哮喘患者外周血在 CTL A4 - Ig作用前后 T细胞表达 IL- 13的情况。结果 :EL ISA法测得哮喘患者IL- 13明显高于正常对照组 (P<0 .0 1) ,CTL A4 - Ig明显抑制了 IL- 13的分泌 (P<0 .0 1)。正常对照组 PHA刺激条件下用逆转录 PCR方法测不出 IL- 13的表达 ;哮喘组在 PHA作用下 ,T细胞表达 IL- 13的量增加 (P<0 .0 1) ;同在 PHA刺激情况下 ,CTL A4 - Ig使 IL- 13表达明显下降 (P<0 .0 1)。结论 :IL- 13在支气管哮喘发病中起重要作用 ,CTL A4 - Ig对 T细胞产生 IL- 13起抑制作用 ,对哮喘的治疗具有临床意义。

Objective To study the expression of IL-13 in T cell from peripheral blood of bronchial asthma patients and the effects of CTLA4-Ig on it in order to study further the pathogenesis of bronchial asthma and to search for a safty and effective route to treat bronchial asthma. Methods The methods of ELISA and RT-PCR were used to determine the expressions of IL-13 in T cell from periferal blood of asthma patients before and after CTLA4-Ig treatment. Results The expression of IL-13 in bronchial asthma patients was significantly higher than normal control group (P<0.01). CALA4-Ig inhibited remarkably the secretion of IL-13 (P<0.01). The expression of IL-13 couldn′t be obtained in normal control group with PHA stimulation, and it was increased in asthma group (P<0.01), and CTLA4-Ig could decrese the exprension of IL-13 obviously after PHA stimulation (P<0.01). Conclusion IL-13 can play an important role in the pathogenesis of bronchial asthma, CTLA4-Ig can inhilit the express of IL-13 in T cell and there is clinical significance for asthma treatment.