p16和p15基因在子宫内膜癌中的表达

Expressions of p16 and p15 genes in uterine endometrial carcinoma

目的 :探讨抑癌基因 p16及 p15在子宫内膜癌、子宫内膜不典型增生及正常子宫内膜组织中的表达及其与子宫内膜癌的关系。方法 :应用免疫组化技术检测 5 7例子宫内膜癌、 8例子宫内膜不典型增生、 7例子宫内膜增生过长及 7例正常增生期子宫内膜组织中抑癌基因 p16、p15的表达。结果 :p16在子宫内膜癌组织及非癌子宫内膜组织中表达率分别为 4 0 .4 %和 77.3% ,二者表达率差异有显著性 (P<0 .0 5 ) ,在子宫内膜癌中 p16表达与病理类型、病理分级及手术 -病理分期有关 ,与肌层浸润无关 ;p15在子宫内膜癌及非癌子宫内膜组织中表达率分别为 4 7.4 %和 86 .3% ,二者比较差异有显著性 (P<0 .0 5 ) ,在子宫内膜癌组织中 p15的表达与病理分级有关 ,与病理类型、肌层浸润及手术 -病理分期无关 ,子宫内膜癌组织中 p16与 p15表达呈正相关 (r=0 .91,P<0 .0 5 )。结论 :p16和 p15表达缺失在子宫内膜癌的发生发展过程中发挥一定作用 ,其中以 p16更为显著 ;p16和 p15的表达具有一致性 ,功能上有一定的互补作用。

Objective To study the expressions of p16 and p15 in human endometrial carcinoma,atypical hyperplasia of endometrium, and normal endometrium and its significances. Methods The expressions of p16 and p15 was examined in 57 specimens of malignant, 8 specimens of borderline,7 specimens of benign uterine endometrial tissues and 7 specimens of normal endometrium by immunohistochemistry S-P methods. Results The positive rates of p16 expression in the malignant and non-malignant tissues were 40.4% and 77.3%, respectively, significant difference was observed (P<0.05). The expression of p16 was associated with pathological types, pathological grades and operation-pathology stages, but no association was found with muscle infiltrations. The positive rates of p15 expression in the malignant and non-malignant tissues were 47.4% and 86.3%, respectively, significant difference was observed (P<0.05). The expression of p15 was associated with pathological grades, but no association was found with pathological types, muscle infiltrations, and operation-pathology stages. There was positive correlation between p16 expression and p15 expression in uterine endometrial carcinoma(r=0.91, P<0.05). Conclusion Low expressions of p16 and p15 may contribute to the development of uterine endometrial carcinoma.