摘要
目的 :探讨 p16基因甲基化在急性淋巴细胞白血病 (AL L)发生机制中的作用。方法 :采用限制性内切酶酶切结合多聚酶链反应方法检测 82例 AL L 患者白血病细胞 p16基因部分启动子和外显子 1Cp G岛甲基化的情况。结果 :31例 AL L 患者存在 p16基因的甲基化 ,1例 p16基因纯合缺失 ;p16存在甲基化与纯合缺失的患者初次化疗完全缓解率和平均生存时间低于 p16基因正常表达者。结论 :p16基因甲基化在 AL L 发生机制中有一定的作用 ,可作为评估 AL L 预后的一个指标。
Objective To investigate the effects of methylation p16 on pathogenesis of actue lymphoblastic leukemia.Methods The restriction endonuclease enzyme digestion combined with PCR was used to detect p16 gene and methylation in 82 patients with acute lymphoblastic leukemia. Results One homozygous deletion was found in these cases and methylation of p16 gene was found in 38.3% patients (31/81) . Patients without methylation of p16 gene had a higher complete remission rate of first chemotherapy and a longer survival time than those with methylation of p16 gene. Conclusion Methylation of p16 gene probably plays a role in the pathogenesis of acute lymphoblastic leukemia and may have a prognostic value.
出处
《吉林大学学报(医学版)》
CAS
CSCD
北大核心
2005年第1期124-126,共3页
Journal of Jilin University:Medicine Edition
基金
吉林省科技厅资助项目 (2 0 0 10 5 35 )