基质金属蛋白酶含量及表达与膀胱癌侵袭转移的关系

Correlation of content and expression of matrix metallo-proteinases in transitional cell carcinoma of bladder with tumor invasion and metastasis

目的 :探讨基质金属蛋白酶 2 (MMP- 2 )、基质金属蛋白酶 9(MMP- 9)的血清含量及组织表达与膀胱移行细胞癌侵袭转移的关系。方法 :用 EL ISA法检测 6 8例膀胱移行细胞癌患者及 2 0例正常对照组血清中MMP- 2和 MMP- 9的含量 ;应用免疫组织化学 SP法 ,检测 6 8例膀胱移行细胞癌、 10例正常膀胱黏膜组织中MMP- 2和 MMP- 9的表达及分布情况。结果 :膀胱癌患者血清中 MMP- 2和 MMP- 9含量明显高于正常对照组(P<0 .0 1) ,淋巴结转移组患者血清 MMP- 2及 MMP- 9含量高于无淋巴结转移组 ;膀胱癌组织中 MMP- 2及MMP- 9表达显著高于癌旁组织及正常黏膜 ,随着肿瘤分级的增高 MMP- 2和 MMP- 9表达显著增强 (P<0 .0 5 )。结论 :MMP- 2和 MMP- 9血清含量及组织表达与膀胱癌侵袭转移有密切关联 ,可以作为判断膀胱移行细胞癌预后的客观指标。

Objective To investigate the correlation of matrix metalloproteinase-2,-9 (MMP-2,MMP-9) in transitional cell carcinoma of bladder (TCCB) with tumor invasion and metastasis. Methods The total serum MMP-2 and MMP-9 levels were detected in 68 patients with TCCB and 20 healthy controls by ELISA; the expresions and distributions of MMP-2 and MMP-9 in 68 patients and 10 normal bladder tissue were determined by immunohistochemical method. Results The levels of serum MMP-2 and MMP-9 were significantly elevated in cancer samples compared with normal (P<0.01), significant difference was found between the patients with metastasis and without metastasis. In TCCB, the expressions of MMP-2 and MMP-9 were increased significantly compared with normal bladder epithelial. The expressions of MMP-2 and MMP-9 were correlated with histological grade and lymph node metastasis (P<0.05). Conclusion These results suggest that the total levels of serum MMP-2 and MMP-9 and their expressions may provide reliable information in TCCB prognosis.