期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

人存活素基因编码区的克隆和序列分析 被引量：1

Cloning and sequencing of human survivin gene
下载PDF
导出
摘要 目的 :克隆人存活素 (survivin)基因编码区cDNA并进行序列分析。　方法 :以人骨肉瘤细胞系MG6 3的mR NA为模板 ,采用RT PCR方法得到人存活素的编码区cDNA ,克隆至载体pUC19中 ,酶切鉴定后进行序列分析。结果 :获得人存活素编码区基因和重组质粒pUC19 SURVIVIN ,DNA序列分析证实所获得的存活素基因编码区cDNA序列和文献报道一致。　结论 :采用基因克隆的方法获得人存活素基因 ,为进一步研究其结构和功能奠定了基础。 Objective:To clone the gene encoding of human survivin. Methods:Using the isolated total RNA from osteosarcoma cell line MG-63 as the template, the cDNA encoding of human survivin was amplified by reverse transcription-polymerase chain reaction (RT-PCR) method, and then cloned into pUC19 and sequenced. Results:The cloned human survivin sequence as the same as reported. Conclusion:Human survivin gene had been cloned successfully. It is useful for the analysis of its structure and function.
作者 陈翔 马保安 杨彤涛 张勇 杨辉 范清宇
机构地区 第四军医大学唐都医院解放军骨科中心骨肿瘤研究所 第四军医大学生物化学教研室
出处 《医学研究生学报》 CAS 2005年第1期17-19,共3页 Journal of Medical Postgraduates
关键词 存活素 基因克隆 序列分析 Survivin Gene cloning DNA sequencing
分类号 Q786 [生物学—分子生物学]
  • 相关文献

参考文献13

  • 1Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma[J]. Nat Med,1997,3(8):917-921.
  • 2杨景,柳凤轩,阎晓初.凋亡抑制因子存活素的研究进展[J].癌症,2003,22(7):771-774. 被引量：25
  • 3Verdecia MA, Huang H, Dutil E et al. Structure of the human anti-apoptotic protein survivin reveals a dimeric arrangement[J]. Nat Struct Biol,2000,7(7):602-608.
  • 4Suzuki A, Ito T, Kawano H et al. Survivin initiates procaspase 3/p21 complex formation as a result of interaction with Cdk4 to resist Fas-mediated cell death[J]. Oncogene, 2000, 19(10):1346-1353.
  • 5Shin S, Sung BJ, Cho YS et al. An anti-apoptotic protein human survivin is a direct inhibitor of caspase-3 and 7[J]. Biochemistry,2001,40(4):1117-1123.
  • 6Suzuki A, Hayashida M, Ito T et al. Survivin initiates cell cycle entry by the competitive interaction with Cdk4/p16(INK4a) and Cdk2/cyclin E complex activation[J]. Oncogene,2000, 19(29):3225-3234.
  • 7Leher R, Enomoto T, McGrath JA et al. Correlation of survivin mRNA detection with histologic diagnosis in normalendometrium and endometrial carcinoma[J]. Acta Obstet Gynecol Scand, 2002, 81(2):162-167.
  • 8蒲小勇,陈一戎,王志平,付生军,卢建中,史庭恺,马宝良.膀胱癌患者尿脱落细胞中survivin基因的检测[J].中国临床康复,2003,7(8):1276-1277. 被引量：10
  • 9Sarela AI, Macadam RC, Farmery SM et al. Expression of the anti-apoptosis gene , survivin ,predicts death from recurrent colorectal carcinoma[J]. Gut,2000,46(5):645-650.
  • 10Trieb K, Lehner R, Stulnig T et al. Survivin expression in human osteosarcoma is a marker for survival[J]. Eur J Surg Oncol,2003 ,29(4):379-382.

二级参考文献11

  • 1Christine M, Michele L, Andrea H, Eduard E, Michael M, Amrin P. Comparison of two qualitative assaye, the ubc rapid test and the BTA star teet, in the diagnosis of mothelial cell carcinoma of the bladder. Urology 2000:56(2):228-31.
  • 2Christine M, Michele LA, Andrea H, Eduard E, Michael M, Amrin P. Comparison of the monoclonal UBC-ELISA test and the NMP22-ELISA test for the detection of urothelial cell carcinoma of the bladder. Urology 2000; 55 (2): 223 -6.
  • 3Vinata B. Can O. Henri T. et al. Urinary hyaluronic acid and hyaluronidase,: markers for bladder cancer detection and evaluation of grade. The Journal of Urology, 2000;163 (1): 248 -356.
  • 4Heicappel R, Schostak M, Mμller M, Miller K. Evaluation of the urinary bladder cancer antigen as a maker for diagnosis of transitional cell carcinoma of the urinary bladder. Scand J Clin Lab Invest 2000: 60(4): 275 - 82.
  • 5Mungan N, Vrisema J, Thomas C, Kiemeney L, Witjes J. Urinary bladder cancer test: a new urinary tumor on the follow-up of supeaficial bladder cancer. Urology 2000:56 (5): 787 - 92.
  • 6Jiang X, Wilford C, Duensing S, Munger K, Jones G, Jones D. Participalion of survivin in mitotic and apoptotic activities of nomal and tumor derived cells. J Cell Biochem 2001 ~,83 (2): 34.2 - 54.
  • 7Suzuki A, Hayashida M, Ito T, et al. Oncogene, Survivin initiates cell cycles entry by the competitive interaction with Cdk4/p16(INK4a) and Cdk2/cyclin E complex activation. Ocogene 2000; 19(29): 3225 - 34.
  • 8Shannon D, Marcia A, Janet Plescia BS, John W, Robert M, Dario C. Urine detection of surnvin and diagnosis of bladder cancer. JAMA 2001 ; 285 (3) ; 324 - 8.
  • 9Sharp JD, Haualaden DA, Maher MG, Wheeler MA, Altien DC, Weiss RM . Front Biosci. Bladder cancer deteotion with urinary survivin, an inhibitor of apoptosis. Front Biosci 2002;7(2): E36 -41.
  • 10王孝养,陈仕新,张珍祥,吕嘉春,方慧娟,熊维宁.survivin基因在非小细胞肺癌中的表达及与P53、c-myc、k-ras蛋白表达的关系[J].中华结核和呼吸杂志,2001,24(6):371-374. 被引量：63

共引文献33

同被引文献10

  • 1刘娟,孙永涛,杜德伟,李光玉,刘秋平,翟嵩.人中性粒细胞多肽1,3真核表达载体的构建与鉴定[J].医学研究生学报,2004,17(10):868-871. 被引量：3
  • 2Chvatchko Y, Proudfoot AE, Buser R, et al. Inhibition of airway inflammation by amino-terminally modified RANTES/CC chemokine ligand 5 analogues is not mediated through CCR3 [ J ] . J Immunol, 2003, 171 (10) : 5498-5506.
  • 3Shahlara S, Proudfoot AE, Woods JM, et al. Amelioration of rat adjuvant-induced arthritis by Met-RANTES [ J ]. Arthritis Rheum, 2005, 52(6) : 1907-1919.
  • 4Crump MP , Rajarathnam K, Kim KS,et al. Solution structure of eotaxin , a chemokine that selectively recruits eosinophils in allergic inflammation [ J ] . J Biol Chem, 1998,273 (35) :22471-22479.
  • 5Mayer MR, Stone MJ. Identification of receptor binding and activation determinants in the N-terminal and N-loop regions of the CC chemokine eotaxin [ J ] . J Biol Chem, 2001, 276 ( 17 ) :13911-13916.
  • 6Akeo S, Mayumi KK, Naoko SN, et al. N-terminal domain of eotaxin-3 is important for activation of CC chemokine receptor 3[J]. Protein Engineering, 2002, 15 ( 11 ) : 923-929.
  • 7Salib RJ, Lau LC, Howarth PH. Nasal lavage fluid concentrations of eotaxin-1 ( CCL11 ) in naturally occurring allergic rhinitis: relationship to disease activity, nasal luminal eosinophil influx, and plasma protein exudation [ J]. 2005, 35 (8) :995-1002.
  • 8Im GJ, Hwang CS, Jung HH. Quantitative expression levels of regulated on activation, normal T cell expressed and secreted and eotaxin transcripts in toluene diisocyanate-induced allergic rats[ J ]. Acta Otolaryngol , 2005 , 125 (4) :370-377.
  • 9Jahnz-Rozyk K,Targowski T, Glodzinska-Wyszogrodzka E,et al.Cc-chemokine eotaxin as a marker of efficacy of specific immunotherapy in patients with intermittent IgE-mediated allergic rhinoconjunctivitis [ J]. Allergy , 2003 , 58 (7) :595-601.
  • 10孙星慧,谭建明,严伟,吴卫真,周浩,林文洪.传染性软疣病毒MC148基因的克隆和序列分析[J].医学研究生学报,2004,17(4):300-301. 被引量：1

引证文献1

二级引证文献3

医学研究生学报
2005年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部