摘要
基因芯片技术是近年兴起的一项重要的生物技术。作者介绍了基因芯片的起源、发展及其基本操作 ,并介绍了铺瓦式阵列、直接等位基因特异性荧光检测法、辅助寡核苷酸链、连接酶检测反应、肽核酸技术等几种不同芯片技术在突变检测中的应用 。
Gene chip is an important biological technology, which is developing quickly recently. In this article, we reviewed the origin, development, basic operation of gene chip. Several kinds of gene chips including tiled array, direct allele specific fluorescence targeting (DAFT) array, labeled auxiliary oligonucleotides array, ligase detection reaction array, and peptide nucleic acid array were introduced as examples for their use in mutation detection. The prospect of the use of gene chip in optimizing drug therapy was also discussed.
出处
《医学研究生学报》
CAS
2005年第1期54-58,共5页
Journal of Medical Postgraduates
基金
国家自然科学基金资助项目 (批准号 :3 0 472 0 5 5 )
关键词
基因芯片
突变检测
合理用药
Gene chip
Mutation detection
Optimizing drug therapy