一株对氯苯胺降解菌的分离鉴定及其降解特性

Isolation and Characterization of a p-Chloroaniline-Degrading Bacterial Strain

从某化工厂污水处理车间好氧池活性污泥中分离到一株降解对氯苯胺的细菌PCA0 39菌株 ,该菌株能够以对氯苯胺为唯一碳源、氮源生长 .经过对其形态特征、生理生化、以及 16SrDNA序列分析 ,该菌株初步鉴定为Diaphorobactersp ..进一步研究表明 ,该菌株的生长过程中 ,氯离子释放同步于对氯苯胺降解 ,并且氯离子的释放量与对氯苯胺的降解量相当 .其利用对氯苯胺生长的最适温度和 pH分别为 30℃和 7 5 ,3d时间内的最适降解浓度为 30 0mg/L(2 35mmol/L) .测定了降解途径中相关酶的活性 ,表明对氯苯胺经过苯胺双加氧酶初始氧化和羟基化后 ,芳环的裂解是由邻苯二酚 2 ,3 双加氧酶催化 .

A bacterial strain that degrades p-chloroaniline is isolated from activated sludge of wastewater treatment plant treating wastewater from a chemical factory. Results indicate that this strain took p-chloroaniline as sole carbon, nitrogen and energy sources. It is identified as Diaphorobacter sp. PCA039 according to its morphology, and biochemical properties, and 16S rDNA sequence analysis. Further study indicate that chloride anion is released simultaneously and stoichemically to p-chloroaniline degradation during cultivation. The optimal pH and temperature for cell growth and p-chloroaniline degradation were 7.5 and 30℃, respectively, and the optimal concentration of p-chloroaniline in three days was 300 mg/L (2.35 mmol/L). Enzymatic analysis show that initial reactions of p-chloroaniline degradation by Diaphorobacter sp. PCA039 are catalyzed by aniline dioxygenase and chlorocatechol 2,3-dioxygenase.