库肯勃瘤组织VEGF,TGF-β_1,MMP-2,MMP-9表达的意义

Expression of VEGF, TGF-β1, MMP-2 and MMP-9 in Krukenberg tumor

目的:研究VEGF,TGF-β1,MMP-2,MMP-9在卵巢原发上皮性肿瘤、库肯勃瘤中的表达及其相关性, 以探讨其在库肯勃瘤发生、发展中的作用. 方法:应用免疫组化S-P法检测VEGF,TGF-β1, MMP-2,MMP-9在45例卵巢原发上皮性肿瘤、38 例库肯勃瘤中的表达情况. 结果:VEGF、TGF-β1在卵巢原发上皮性肿瘤和库肯勃瘤组织中的表达显著高于正常卵巢组织(VEGF: x2=5.318,P=0.021;x2=22.985,P=0.001. TGFβ1:x2=9.778,P=0.002;x2=12.584,P=0.001). MMP-2,MMP-9在正常卵巢组织中表达缺如; VEGF,MMP-2,MMP-9在库肯勃中阳性表达率明显高于卵巢原发上皮性肿瘤(VEGF:x2=13.149,P=0.001; MMP-2:x2=33.668,P=0.001;MMP-9:x2=38.839, P=0.001);TGF-β1阳性表达率在卵巢原发上皮性肿瘤和库肯勃瘤之间无显著差异.VEGF,TGF-β1, MMP-2,MMP-9在卵巢原发上皮性肿瘤、库肯勃瘤中,任意两指标阳性表达均有正相关性(P<0.05或P<0.01). 结论:VEGF,TGF-β1,MMP-2,MMP-9参与卵巢癌、库肯勃瘤的发生、发展及演进过程,并可为卵巢上皮性肿瘤及库肯勃瘤的鉴别诊断提供一定依据.

AIM: To examine the expression of vascular endothelial growth factor (VEGF), transformation-growth factor-β1(TGF-β1), matrix metalloproteinase (MMP)-2 and MMP-9 in primary epithelial ovarian carcinoma and Krukenberg tumor, and to explore their effects in the carcinogenesis and development of Krukenberg tumor. METHODS: The expression of VEGF, TGF-β1, MMP-2 and MMP-9 was measured by immunohistochemical S-P method in 45 cases of primary epithelial ovarian carcinoma and 38 cases of Krukenberg tumor. RESULTS: The positive rates of VEGF and TGF-β1 expression were significantly higher in the tissues of primary epithelial ovarian carcinoma and Krukenberg tumor than those in the normal ovarian tissues (VEGF: x2 = 5.318, P = 0.021; x2 = 22.985, P = 0.001; TGFβ1:x2 = 9.778, P - 0.002;x2 = 12.584, P = 0.001). The positive rates of MMP-2 and MMP-9 expression were zero in the normal ovarian tissues. The expressions of VEGF, MMP-2 and MMP-9 were remarkably higher in the Krukenberg tumor than those in the primary epithelial ovarian carcinoma (x2 = 13.149, P = 0.001; x2 = 33.668, P = 0.001; x2 = 38.839, P = 0.001). The positive expression of TGF-β1 was not significantly different between tissues of primary epithelial ovarian carcinoma and Krukenberg tumor. The expression of VEGF, TGF-β1,MMP-2 and MMP-9 positively correlated with one another in primary epithelial ovarian carcinoma and Krukenberg tumor (P<0.05 or P<0.01). CONCLUSION: VEGF, TGF-β1, MMP-2 and MMP-9 play important roles in the carcinogenesis and development of ovarian carcinoma and Krukenberg tumor. They may provide evidence for the differential diagnosis between the two diseases.