喉鳞状细胞癌癌细胞的原代培养

A study on primary culture in vitro of twenty-one laryngeal squamous carcinoma tissues

目的:探讨喉鳞状细胞癌组织原代培养的细胞特性、生长状况及生长条件,比较胶原酶消化培养与 胰蛋白酶消化培养对原代细胞的影响,为喉鳞状细胞癌细胞的原代培养研究提供实验基础。方法:应用细胞培养 技术,对21例人喉鳞状细胞癌组织分别用胶原酶消化和胰蛋白酶消化进行体外培养,其培养基加有5%(V/V) 低浓度胎牛血清的RPMI1640,观察人喉癌细胞生长情况。结果:胶原酶消化培养优于胰蛋白酶消化培养,胶原 酶消化培养细胞生长率为58%,胰蛋白酶消化培养细胞生长率为13%(χ2=7.048,P<0.05),低浓度血清培养 基可抑制成纤维细胞的生长。结论:胶原酶消化培养优于胰蛋白酶消化培养,喉癌细胞培养的关键是防止污染及 成纤维细胞的过度生长。

Objective:To study the different influent on the growth rate of laryngeal carcinoma cell culture in vitro between collagenase type I and typsin and to provide the experiment base for laryngeal carcinoma cell culture.Method:Applying the technique of primary tissue culture in vitro, twenty-one specimens which were identified pathologically as laryngeal squamous cell carcinoma were cultured. The relation ship letwech the cell growth rate and donor age,cultured methods was observed.The influent factors of primary culture,such as serum of low concentration were analyzed.Result:Cell growth rate were 66%(6/9) in the group aged under 60, 58% in the group above 60.The cell growth rate were 58% in collagenase type I and 13% in typsin. Serum of low concentration can restrain fibroblast.Conclusion:The cell growth of laryngeal squamous cell carcinoma in vitro could be correlated with the culture methods, fibroblast outgrowth and microbiotic contamination are important factors in primary cell culture.