外源性供氧和超氧化物歧化酶对脊髓损伤的保护作用

The Prtective Effect of Exogenous Oxygen and Superoxide Dismutase on Acute Spinal Cord Injury

目的 观察外源性供氧和超氧化物歧化酶 (SOD)保护受损伤脊髓 ,促进其功能恢复的作用。方法 采用Allen’s法于兔L2 椎体水平造成急性脊髓损伤。对照组不予治疗 ,治疗组分别在伤后 3 0min开始给药。于伤后 12h ,测定脊髓组织丙二醛 (MDA)及SOD活性 ,四周后进行病理形态学观察。结果 联合应用外源性供氧和SOD ,在抑制MDA生成 ,增强SOD活性方面较单一用药显著 (P <0 0 5 )。伤后四周末外源性供氧组 (2 9± 1 1)级 ,SOD组 (2 8± 1 2 )级 ,联合用药组 (3 1± 0 8)级 ,对照组 (1 1± 0 9)级 ,三个治疗组的肌力恢复均好于对照组 (P <0 0 5 )。组织形态学检查联合用药组的病理改变较单一用药组轻。结论 脊髓损伤后早期外源性供氧和SOD协同作用 ,能有效抑制脊髓损伤后的脂质过氧化反应 ,对继发性脊髓损伤有保护作用。

Objective To investigate the protective effect of exogenous oxygen and superoxide dismutase (SOD) on acute spinal cord injury. Methods A rabbit model of L2 spinal cord injury was produced by Allen's method, and then the rabbits were randomly divided into experimental group and control group. In the experimental group, the rabbits received exogenous oxygen, SOD or both 30 min after injury. 12h after injury, the amount of MDA and SOD of spinal tissues were determined, and pathological changes of spinal cord were observed at the fourth weeks after injury. Results Combinant administration of exogenous oxygen and SOD could inhibit MDA production and increase SOD activities, effect of which was stronger that single administration (P<0.05). The muscle force recovery of lower extremity was better in the treatment group than that in the control group (P<0.05). The pathological lesions of spinal cord in the rabbits treated by combination administration were milder that those in the rabbits treated by single administration. Conclusion Combinant administration of SOD and exogenous oxygen could inhibit lipid peroxidation after spinal injury and had protective effects on the secondary spinal cord injury.