抗凋亡基因bag-1和bcl-2在结肠癌组织中的表达及其相互关系

Expression of anti-apoptotic gene bag-1 and bcl-2 in colorectal cancer and their interations

目的 研究抗凋亡基因bag 1和bcl 2在结肠癌组织中的表达及其意义 ,并探讨两者间的关系。方法 应用免疫组织化学链霉亲合素 生物素 过氧化物酶复合物法 (SABC法 )对 64例结肠癌组织及 10例正常结肠组织中bag 1和bcl 2基因的表达进行检测。 结果 bag 1和bcl 2在结肠癌中的表达与结肠癌病理类型、肿瘤直径、浸润深度及有无淋巴结转移无关 ,但bag 1与病理分级、远处转移、Dukes分期及预后密切相关 (P <0 .0 5 ) ,而bcl 2与这几项临床病理因素无明显相关性 (P >0 .0 5 ) ;bag 1和bcl 2在结肠癌的发生发展过程中呈正相关(r =0 .475 )。 结论 结肠癌组织中有不同水平bag 1和bcl 2蛋白的高表达 ,它们可作为结肠癌早期筛选的一项指标 ,并对疾病的预后有着重要意义。

Objective To investigate the expression of bag-1 and bcl-2 in colorectal cancer and evaluate their clinical significance.Methods The expression levels of bag-1 and bcl-2 were detected by using immunohistochemical Streptavidin-Biotin-Peroxidase Complex (SABC) method in 64 samples of colorectal cancer tissues,10 normal colorectal tissues.Results bag-1 was positively related to the tumour grade,distant metastasis,stages of Dukes and prognosis,but not to the pathological cell types,tumour diameter,depth of invasion and lymph node metastasis.bcl-2 was not correlated to these clinicopathologic factors.There was a positive correlation between bag-1 and bcl-2 (r=0.475).Conclusion There is overexpression of bag-1 and bcl-2 protein in colorectal cancer.bag-1 and bcl-2 might serve as a biomarker to diagnose the early colorectal cancer.