贵州从江侗族Y-DNA及线粒体DNA序列多态性分析

The Sequence Polymorphism of Y-chromosome DNA and mtDNA of Dong Ethnic of Congjiang, Guizhou

为分析贵州从江侗族父系及母系遗传结构,探讨其起源及迁徙,通过聚合酶链式反应 限制性片段长度多态性(PCR RFLP),研究贵州从江侗族无亲缘关系个体由10个单核苷酸位点(SNPs)组成的Y染色体单倍型及11个单核苷酸位点组成的线粒体DNA单倍群频率。结果显示,从40份男性样本的Y SNP基因分型中,得到H6、H11、H14共3种单倍型;H11的频率为92.5%;通过对线粒体DNA基因分型,得到6种单倍群,有75%的个体能明确分类其所携带的单倍群特征,说明贵州从江侗族父系遗传构成相对简单。通过主成分分析,证明贵州从江侗族与其他的壮侗语族人群相聚,母系遗传结构复杂,无C单倍群分布可能为该民族特征之一。

In order to explore the patrilineal and matrilineal genetic structure and the origin of Dong Ethnic of Congjiang in Guizhou province, the distribution of Y-chromosome haplotypes which consisted of 10 SNPs of Y-DNA and mtDNA haplogroups consisted of 11 SNPs were studied by using PCR-RFLP method.The result showed three haplotypes H6,H11,H14 were detected, the frequency of H11 is 92.5%.Six haplogroups were identified by mtDNA analysis, 75% of the people can be identified.The patrilineal genetic structure of Dong of Guizhou is simple, principle component indicated that the structure is closer to Zhuang-Dong branch of Sino-Tibetan language family.The matrilineal genetic structure of Dong of Guizhou is complicated.