利用微卫星标记进行凉山半细毛羊亲权鉴定的研究

Microsatellite Markers for Paternity Testing of Liangshan Semi-fine Wool Sheep

经多重PCR扩增20个微卫星标记,对凉山半细毛羊进行亲权鉴定。荧光标记检测扩增产物,并用Cervus2.0分析。结果表明:当双亲未知时,累积排除概率为0.998666,在父系半同胞基础上进行母子亲权鉴定,累积排除概率为0.999994,置信度为95%,最终从8个候选父亲中找出215个后裔的父亲,又从147个候选母亲中找出它们的母亲,构建了一个混合家系系谱,为进行连锁分析、QTL定位奠定了基础,并对研究绵羊群体结构、历史及多样性有重要意义。

The usefulness of multiplex polymerase chain reaction was evaluated and fluorescent detection with 20 microsatellites was used for paternity testing of Liangshan semi-fine wool sheep. The results in Cervus 2.0 indicated that the combined exclusion probability was 0.998666 when parents were both unknown. If father was known, combined exclusion probability was 0.999994, with confidence level of 95%. Finally, the biological parents of 215 individuals in offspring were found from eight candidate fathers and 147 candidate mothers, and a mixed pedigree was constructed. It is useful for linkage analysis of sheep chromosomes and QTL location, also for the study of population structure, history, and diversity.