中国蒙古马与国外纯血马mtDNA D-Loop高变区序列比较

Sequence Comparing of mtDNA D-Loop Varied Region in Chinese Mongolian Horse and External Thoroughbred Horse

比较分析了4匹中国蒙古马和4匹国外纯血马的线立体DNA(mtDNA)D Loop高变区400bp核苷酸序列的变异情况。结果发现,4匹中国蒙古马mtDNAD Loop高变区的平均核苷酸变异率为3 69%,而纯血马的为4 00%,其核苷酸变异类型均包括转换、颠换和缺失3种形式,其中以转换最为常见。核苷酸变异基因座多,并且存在长度变异,不同变异在个体之间差异也很大,因此说明中国蒙古马和国外纯血马的mtDNAD Loop高变区都具有丰富的多态性。

Mitochondrial DNA D-Loop varied region 400 bp sequence variations in four Chinese Mongolian horses and four External Thoroughbred horses were analyzed in this experiment. The results showed that the average nucleotide mutational rate of mtDNA D-Loop varied region in four Chinese Mongolian horses was 3.69%,while External Thoroughbred horses were 4.00%. Three types of mutations including transition,transversion and deletion were all found in the investigated mtDNA D-Loop regions,of which transition was the most frequent. Nucleotide mutational loci were abundant,length mutations were found and great differences were all observed among the eight horses. It showed there existed much polymorphism in the mitochondrial DNA D-Loop varied region of Chinese Mongolian horses and External Thoroughbred horses.