现场紫外光谱及圆二色谱研究电化学还原反应诱导微过氧化物酶-11构象的转变

In-situ UV and CD Spectroelectrochemistry Investigation of the Electrochemical Reduction Reaction Inducing Conformational Transition of Microperoxidase-11

采用现场紫外光谱及圆二色谱电化学方法研究了微过氧化物酶 -1 1的电化学还原过程 .同时应用奇异值分解最小二乘法和双对数法对所得光谱数据进行处理 .研究发现 ,电化学还原过程诱导微过氧化物酶 -1 1的构象由无规卷曲向 α螺旋转变 ,这为进一步理解生物电子传递过程与生物分子构象转变机理提供了基础信息 .

Electrochemical reduction process of microperoxidase-11 was investigated by in-situ UV-Vis and CD spectroelectrochemistry methods with a long optical path thin layer cell. The spectra obtained were analyzed by singular value decomposition least square and double logarithmic analysis methods. The results showed that the electrochemical reduction induced the conformational transition of MP-11 from random coil to α-helix and provided an important information for understanding biological electron transfer mechanism coupled with conformational transitions.