摘要
采用现场紫外光谱及圆二色谱电化学方法研究了微过氧化物酶 -1 1的电化学还原过程 .同时应用奇异值分解最小二乘法和双对数法对所得光谱数据进行处理 .研究发现 ,电化学还原过程诱导微过氧化物酶 -1 1的构象由无规卷曲向 α螺旋转变 ,这为进一步理解生物电子传递过程与生物分子构象转变机理提供了基础信息 .
Electrochemical reduction process of microperoxidase-11 was investigated by in-situ UV-Vis and CD spectroelectrochemistry methods with a long optical path thin layer cell. The spectra obtained were analyzed by singular value decomposition least square and double logarithmic analysis methods. The results showed that the electrochemical reduction induced the conformational transition of MP-11 from random coil to α-helix and provided an important information for understanding biological electron transfer mechanism coupled with conformational transitions.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2005年第2期321-325,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :2 0 2 75 0 3 6
2 0 2 75 0 3 7)资助
关键词
微过氧化物酶-11
光谱电化学
电子传递
构象变化
Microperoxidase-11
Spectroelectrochemistry
Electron transfer
Conformation change