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家兔盲肠微生物总DNA的提取 被引量：9

Extraction of Rabbit Cecum Microbial Total DNA
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摘要 为了全面了解家兔盲肠中的微生物信息,本文采用非培养技术,通过化学、物理裂解和酶解相结合,建立了直接从家兔盲肠内容物中提取总DNA的方法。结果从1g新鲜的盲肠内容物中提取出5.4μg总DNA;所得的DNA能被核酸限制性内切酶降解;经纯化后能用作模板进行PCR扩增。 A method of extracting total DNA from rabbit cecum was developed, and 5.4 μg of DNA was extracted form 1 gram of fresh sample. The DNA can be digested by Sau3AⅠ, and can be used as template for PCR after being purified.
作者 文红秀 李昌平
机构地区 西南农业大学动物科技学院 永川朱沱镇畜牧兽医站
出处 《动物科学与动物医学》 2004年第12期28-29,共2页 Animal Science & Veterinary Medicine
关键词 家兔盲肠 微生物 总DNA 提取 rabbit cecum microorganisms total DNA extraction
分类号 S852.6 [农业科学—基础兽医学]
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参考文献7

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二级参考文献12

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共引文献147

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二级引证文献49

动物科学与动物医学
2004年 第12期

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