连续监测法测定腺苷脱氨酶及临床应用

Determination of Adenosine Deaminase by Continuous Monitoring Method and its Clinical Application

目的探讨应用连续监测法测定腺苷脱氨酶(ADA)及其临床应用价值.方法根据测定条件的优化实验采用ADA作用于以腺苷为底物的连续监测法直接测定;同时测定200例健康人、32例肝炎病人血清及13例结核性穿刺液的ADA水平.结果批内CV＜4.0%,批间CV＜4.7%,总CV＜4.3%,回收率达101.3%,线性:0～192 U/L(Y=0.9846X+1.1462,r=0.996 8);加入TG＜5.6 mmol/L,BIL＜165 μmol/L,Hb＜100g/L,UA＜420μmol/L时无显著干扰;血清正常参考范围(-x±s)3～25 U/L;肝疾病组血清ADA水平显著高于健康对照组(P＜0.001),结核病组穿刺液ADA水平显著高于非结核病组(P＜0.001).结论该法简便、准确、快速,对肝炎疾病及结核性穿刺液有一定的诊断价值.

Objective To study the continuous monitoring method of adenosine deaminase (ADA) and its clinical application. Methods Basing on the condition of optimization experiment adopted direct kinetic assay of ADA was studied with adenosine as substrate. Serum ADA levels of 200 normal persons and 32 hepatitis disease were measured;Puncture fluid ADA levels of 13 tuberculosis were measured. Results Within-run CV<4. 0% ,between-run CV<4. 7% and total CV< 4. 3%;The average rate of recovery was 101. 3%;The linearity reached 192 U/L (Y=0. 984 6X+1. 146 2,r=0. 996 8), Adding triglyceride (TGX5. 6 mmol/L,bilirubin(BIL)<165 μmol/L;hemoglobin(Hb)<100 g/L and uricacid(UAX 420 μmol/L without interference;The reference range of serum ADA of normal persons was from(x±s) 3 to 25 U/L, serum ADA levels of hepatitis disease was higher than group of normal persons (P<0. 001),Puncture fluid ADA levels of tuberculosis was higher than no tuberculosis (P<0. 001). Conclusion The method is simple,accurate and easy for differential diagnostic value of hepatitis disease and tuberculosis.