经颈内动脉冲洗治疗急性脑缺血大鼠的实验研究

Experimental Research of Flushing via ICA in Rats with Acute Cerebral Ischemia

目的 :观察经颈内动脉冲洗法对急性脑缺血大鼠脑损伤程度的影响。方法 :采用改良的MCAO方法造模 ,MCA梗阻 2h后 ,冲洗组于再灌注超早期经颈内动脉分别注入 3ml生理盐水或中药溶液 ,对照组于腹腔内注入等体积中药溶液。再灌注12、2 4和 4 8h ,测定大鼠神经功能缺损评分。再灌注 4 8h ,测定脑梗死体积。结果 :缺血再灌注 4 8h后 ,和模型组相比 ,中药冲洗组和腹腔给药组脑梗死体积明显缩小 ,神经功能缺损明显减轻。中药冲洗组和腹腔给药组相比 ,两组在脑梗死体积和神经功能缺损评分上有显著差异。结论 :(1)经颈内动脉冲洗法减轻急性脑缺血后的脑损伤程度作用要优于腹腔给药法。 (2 )经颈内动脉冲洗法对于急性脑缺血后的脑损伤保护作用与不同药物有关 ,祛风通络的中药具有减少急性缺血性大脑损伤程度的作用。

Objective: To study effect of flushing via I CA on rats with acute cerebral ischemic injury. Methods: Using a modified MCAO method, the authors established the model and flushed the ischemi c territory via ICA with 3ml saline(sflush) and Chinese recipe solutions(cflush) respectively at the early start of reperfusion that 2 hours after MCAO. Rats of control(ip group) were given 3ml Chinese recipe solutions ip.at the right time. Cerebral infarct volume was assayed at 48h after reperfusion ,and neurological deficit score was observed at different points after reperfusion. Result s: Compared to model group, 48 hours after reperfusion, cerebral infarct volume of cflush and ip group decreased significantly and neurological deficit scores were increased. It was significantly between cflush and ip group on cereb ral infarct volume and neurological deficit score. Conclusion:(1 ) The effect of flushing via ICA on decreasing acute cerebral ischemic-reperfusi on injury was better than that of treating via ip. (2) The effect of flushing vi a ICA was associated with Qufengtongluo Chinese recipe , which could reduce isch emic-reperfusion injury.