尾加压素II与支气管哮喘相关性的研究

Couelative Evaluation of Urotensin II to Bronchial Asthma

目的 :探讨血清中尾加压素II水平与支气管哮喘的相关性。方法 :选择支气管哮喘患者 2 6例和正常人 2 6例 ,用斑点蛋白印迹法测定样本血清中尾加压素II的水平 ,通过薄层扫描技术和统计学处理 ,对结果进行半定量分析。结果 :支气管哮喘组血清尾加压素II水平 (0 .136± 0 .0 18光密度值 )高于正常对照组 (0 .0 4 8± 0 .0 2 2光密度值 ) ;血清尾加压素II与支气管哮喘显著相关 (P <0 .0 0 1) ;正常对照组的男性与女性之间 ,血清尾加压素II水平无显著差异 (P >0 .0 5 )。结论 :血清中尾加压素II水平与支气管哮喘显著相关 ;尾加压素II可能参与支气管哮喘的发病过程或为支气管哮喘的主要病理改变之一 ;提示尾加压素II效应模式可能是通过血液循环作用于靶器官。

Objective: This study is aimed at investig at ing correlation of Urotensin (UII) in serum to bronchial asthma. Methods : The serum samples were of 52 cases collected from patients with bronch ial asthma and health volunteers as normal controls, undes equally divided memba s for each of both groups. Urotensin II levels in the serum were detected with D ot-Western blot, and semi-quantification analysis had been carried out with Thin Layer Scanner and statistics. Results: Urotensin II levels in t he serum of bronchial asthma group (0.136±0.018) was significantly higher than those normal up the control (0.048 ±0.022) (P<0.001). However, there were n ot significant difference of Urotensin levels between males and females in the n ormal samples from health volunteers(P>0.05). Conclusion:U rotensin II level in the patient serum is correlated to bronchial asthma symptom strikingly. High level of Urotensin II is probably involved in Bronchial asthma developing course of bronchial asthma or is one of main causes for the patholog ical changes. (The above results suggest that effect mode of Urotensin II is exe rting its action on target organs by blood circulation)