肝癌组织中肠道病毒表达的初步研究

Preliminary Study on the Expression of Enterovirus in Hepatocellular Carcinoma

目的研究肠道病毒在肝癌及非肝癌组织中的表达。方法 :采用增强免疫组化方法检测 11例肝细胞性肝癌和 6例非肝细胞性肝癌病人的肝脏组织中肠道病毒的表达情况。并比较了肝癌与癌周组织中肠道病毒表达的差异。结果 :肝细胞性肝癌组阳性率 5 4 .5 4 % ,非肝细胞肝癌组阳性率 0 % ,两者之间有显著差异 (P =0 .0 4 3)。 11例肝细胞肝癌中 ,6例癌组织肠道病毒表达阳性 ,癌周围组织中肠道病毒表达 3例阳性 ,两者之间无显著差异 (P =0 .387)结论 :肝癌病人存在肠道病毒感染。EV感染是以整个肝脏组织为靶器官 ,与肝癌组织无特殊亲和力。EV与肝硬化无关。

Objective:To detect the expression of e nter ovirus (EV) in hepatocellular carcinoma (HCC) and non-HCC. Methods: 17 Surgical specimens of patients with HCC or non-HCC resection during 1999～2000 were used to determine the immunoreactivity of VP1 protein of enterov i rus by enhanced immunohistochemistry. These specimens were divided into HCC grou p (G1) and non-HCC group (G2), according to whether VP1 of enterovirus was expre ssed, the relations between two groups were analyzed and the expression between edge of cancer and cancer were compared. Results: The posi ti ve rates in G1 and G2 were: 54.55%(6/11) versus 0%(0/6) for VP1 protein of ent erovirus. Significant differences were found between 2 groups. The positive rate s within G2 were 54.55% (6/11) in cancer versus 27.28% (3/11) in edges of cance r. there are no significant differences between them. Conclusion: There were no EV expression in non-HCC. EV protein was expressed in the whol e liver in HCC and EV is not related to cirrhosis.