摘要
目的探讨大鼠心肌缺血动物模型的构建。方法缝扎大鼠冠脉左前降支,于左室前外侧壁形成缺血区域,约占左室壁面积的20%～50%。结果完成85例动物模型制作,存活74只,其中60只据术中所见、心电图、及病理检查证实有明确的心肌缺血,心功能下降。结论该方法制作简单可行,动物存活率满意;但模型欠稳定,需标本量较大以充分筛选。
Objective To discuss the method of establishment of cardiac ischemic model in rats. Methods The left anterior descending coronary artery (LAD) was ligated to form an ischemic area which located at the anterolateral left ventricular free wall, accounting for 20%-50% of it. Results\ 85 animals' LAD were stitched, and 74 survived. Among these survived animals, 60 with heart dysfunction were confirmed as qualified ischemic model by operative observation, electrocardiogram and histopathology. Conclusion\ This method can easily create the ischemic heart model with acceptable death rate, but the model's stability is varying, which requires more candidate samples.
出处
《中国实验动物学报》
CAS
CSCD
2004年第4期226-230,F003,i001,共7页
Acta Laboratorium Animalis Scientia Sinica
基金
湖南省科技计划项目(03TCY2012)。
