广东人低分子量多肽基因多态性与变应性鼻炎相关性研究

Study on the Correlation between Polymorphism of Low Molecular Weight Peptide Genes and Allergic Rhinitis in Cantonese

目的 :探讨广东人变应性鼻炎 (allergicrhinitis,AR)患者中低分子量多肽 (lowmolecularweightpepitide ,LMP)基因多态性与变应性鼻炎相关性。方法 :利用聚合酶链式反应技术对 6 9例广东籍变应性鼻炎患者及 10 0例正常对照者的低分子量多肽基因LMP2和LMP7进行扩增 ,并以HhaⅠ酶进行限制性酶切图谱分析。结果 :①变应性鼻炎患者LMP7A/A频率占 8.70 % ,低于正常对照组的 2 1.35 % ,差异有显著性 (P <0 0 5 ) ,其余各型与正常对照组差异无显著性 (P >0 .0 5 )。②变应性鼻炎病人中LMP7等位基因A的频率占 36 .2 3% ,B的频率占 6 3.77% ,与正常对照组的A为 4 7.0 9%、B为 5 2 .91%差异有显著性 (P <0 .0 5 )。结论 :提示LMP7等位基因A与AR的发病呈负关联 ,B与AR的发病呈正关联。

Objective:To study the correlation between polymorphism of low molecular weight pepitide (LMP) genes and allergic rhinits (AR) in Cantonese. Methods: Among 69 AR patients and 100 health controls, who were both Cantonese,LMP2 and LMP7 genes were amplified by polymerase chain reaction (PCR),followed by restriction endonuclease analysis with HhaⅠ. Results: 1) LMP7A/A in AR patients had a prevalence of 8.7%, and was significantly lower than health controls. 2) Compared with 47.09% of LMP7 allelic genes A and 52.91% of LMP allelic genes B in health controls, the patients had significantly different rate with 36.23% of LMP allelic genes A and 63.77% of LMP allelic genes B. Conclusion: The results showed a negative correlation between Lmp/A and AR, and a positive correlation between LMP/B and AR.