P-糖蛋白、GST-π表达预测膀胱移行细胞癌化疗后复发的价值

The value of multiple drug resistance protein P-gp,GST-π in predicting recurrence of patients with bladder transitional cell carcinoma (TCC) after chemotherapy

目的探讨多药耐药蛋白(P-糖蛋白,P-gP)、谷胱甘肽-S-转移酶(GST-π)预测膀胱移行细胞癌 (transitional cell carcinoma,TCC)化疗后复发的价值。方法将51例TCC患者按分级、分期、复发分组,免疫组 化法检测其病理组织标本中P-gP、GST-π表达情况。结果P-gP阳性表达率分别为G1级33.3％(4／12)、G2级 56.7％(17／30)、G3级66.7％(6／9),三级间比较P均>0.05;GST-π阳性表达率分别为G1级25％(3／12)、G2级 66.7％(20／30)、G3级77.8％(7／9),三级间比较P均<0.05。浅表性肿瘤与浸润性肿瘤的P-gP阳性表达率分别 为56.3％(18／32)、47.4％(9／19);GST-π阳性表达率分别为56.3％(18／32)、63.2％(12／19);P均>0.05。无复 发组和复发组P-gP阳性表达率分别为37.5％(9／24)、66.7％(18／27),P<0.05;GST-π阳性表达率分别为50％ (12／24)、66.7％(18／27),P>0.05。结论P-gP表达可作为判断TCC是否复发的指标,GST-π的表达与肿瘤的 分化程度有密切关系。

Objective To determine the value of mutiple drug resistance protein P-gp、GST-π in recurrence of patients with bladder transitional cell carcinoma (TCC).after chemotherapy. Methods Immunohis-tochemical analysis for P-gp,GST-π was performed using streptavidin biotin horseradish peroxidase staining in 51 patients with TCC which were classified by grade,stage and recurrence. Results G1,G2 and G3 group had P-gp, GST-π over-expression respectively in 4/12(33. 3%) , 3/12 (25%), 17/30(56. 7%) , 20/30 (66. 7%),6/9(66. 7%), 7/9(77. 8%). Only GST-π expression had significant difference (P<0. 05). Superficial tumors had P-gp, GST-π overexpression of 56. 3%(18/32) ,56. 3(18/32) while-invasive tumors had P-gp,GST-π overexpression of 47.4%(9/19),63. 2%(12/19) respectively,there were no difference (P>0. 05).Free recurrence group had P-gp,GST-π overexpression in 37.5% (9/24),50% (12/24) respectively, while recurrence group had P-gp,GST-π overexpression of 50% (12/24), 66. 7%(18/27) respectively (P<0. 05). Conclusion P-gp can estimate if the TCC of bladder is recurrent, GST-π can predict differentiation of TCC.