中国北方汉族人群HLA-A、HLA-B、HLA-DR等位基因频率检测及其临床意义

Clinical value of allele frequenly of HLA-A, HLA-B, HLA-DR in the hans of North China

目的从基因水平了解中国北方地区汉族人群人类白细胞抗原HLA-A、HLA-B、HLA-DR位点的 等位基因(以下分别简称为A等位基因、B等位基因及DR等位基因)频率,获得更完整、准确的HLA群体遗传学 数据。方法 应用聚合酶链反应一序列特异性引物(PCR-SSP)方法对2000名北方汉族健康志愿者进行A、B、DR 等位基因分型。结果鉴定了17个A等位基因,32个B等位基因,13个DRB1等位基因。最常见的基因型分别 为A*02、B*13、DRB1*15,其相应基因频率范围分别为0.2400-0.2767、0.1330-0.1432和0.1557-0.1707。结 论 本结果可作为我国HLA多态性研究的群体资料和正常参考值,对群体遗传、疾病关联研究以及寻找HLA 相合的异基因造血干细胞供者具有重要意义。

Objective To explore allele frequency of HLA-A,HLA-B,HLA-DR in the Hans of North China and obtain more comprehensive and accurate data on HLA molecular genetics. Methods Polymerase chain reaction-sequence specific primers(PCR-SSP) were used to determine HLA-A,HLA-B,HLA-DR alleles in 2000 unrelated healthy Han individuals of North China. Results 17 A,32 B and 13 DRB1 alleles were detected. The most common HLA-A,HLA-B,DRB1 type is A* 02,B*13,DRB1* 15 with the frequency of 0. 2400-0. 2767,0. 1330-0. 1432 and 0.1557- 0. 1707 respectively. Conclusion It's important to the study on population genetics and disease associated,and is helpful for potential receipts to find optimal donors.