牛磺酸对顺铂引起的小鼠肾髓质ATP酶活性变化的影响

Influence of taurine on the changes of ATPases activity induced by cisplatin in renal medulla of mice

目的 :探讨牛磺酸对顺铂引起的小鼠肾髓质ATP酶活性的影响 ;方法 :4 0只小鼠随机分为正常对照组、CDDP组、牛磺酸 2 0 0mg组 (Tau2 0 0 )和牛磺酸 4 0 0mg组 (Tau4 0 0 ) ,分取肾髓质、制备匀浆 ,用生化法测定Na+ ,K+ -ATP酶、Ca2 + -ATP酶和Mg2 + -ATP酶活性 ;结果 :CDDP组、Tau2 0 0组、Tau4 0 0组肾髓质Na+ ,K+ -ATP酶、Ca2 + -ATP酶和Mg2 + -ATP酶活性均明显低于正常对照组 (P <0 .0 1)。Tau2 0 0组、Tau4 0 0组肾髓质Na+ ,K+ -ATP酶和Ca2 + -ATP酶活性均明显高于CDDP组 (P <0 .0 1或P <0 .0 5 ) ,而Mg2 + -ATP酶活性与CDDP组相比无显著差别 (P >0 .0 5 ) ;结论 :牛磺酸可明显增加肾髓质Na+ ,K+ -ATP酶和Ca2 + -ATP酶活性 ,对顺铂引起的肾毒性具有防治作用。

Objective:To explore the influences of taurine on the changes of ATP ases activity induced by cisplatin in renal medulla of mice.Methods:40 mice were randomly divided into four groups such as control group,CDDP group,Tau 200 group and Tau 400 group,and the tissue of renal medulla was isolated and the activities of Na +,K + -ATPase ,Ca 2+ -ATPase and Mg 2+ -ATPase were assayed by biochemistry.Results:The activities of Na + ,K + -ATPase,Ca 2+ -ATPase and Mg 2+ -ATPase were significantly decreased in CDDP group,Tau 200group and Tau 400 group when comparing with control group( P <0.01),the activities of Na +,K +-ATP ase and Ca 2+ -ATP ase in Tau 200 group and Tau 400 group were higher than it in CDDP group( P <0.01 or P <0.05),but the activities of Mg 2+ -ATPase in Tau 200 group and Tau 400 group were no different when comparing with CDDP group( P >0.05).Conclusion:Taurine might have the preventive effect on cisplatin induced-nephrotoxicity by obviously increasing the activities of Na + ,K + -ATPase and Ca 2+ -ATPase in renal medulla tissue of mice.