血清10型鸭疫里默氏菌4个亚型的分析

Four Subtypes within Serotype 10 of Riemerella anatipestifer Isolates from Ducks in China

采用琼脂扩散沉淀试验,对只与血清10型鸭疫里默氏菌参考菌株H2199的抗血清发生凝集反应的 58 株分离株进行了抗原性分析。根据沉淀反应模式,可将这些分离株分成4类:第1类是以 C2 为代表的 30 株;第 2 类是以C449为代表的3株;第3类是以 C459 为代表的 6 株;第 4 类是以 C598 为代表的 19 株。第 1 类分离株与H2199具有相同的热稳定抗原模式,其他类型的分离株与各类代表菌株的热稳定抗原具有同一性,但彼此之间以及与H2199之间存在明显的抗原差异。用未经吸收和经过吸收的抗血清经玻片凝集试验和试管凝集试验对H2199和4类分离株彼此之间的抗原关系进行了进一步的分析。以 C2 为代表的分离株属于真正的 10 型;以C459为代表的分离株与H2199存在显著的抗原差异,它们除了与 H2199 拥有共同抗原外,还有自己的特异性抗原成分,因此,H2199与C459之间的抗原关系可描述为 ab- bc。类似的抗原关系还分别存在于 H2199 与 C598、C598与C449之间。但是,在试管凝集试验中,分别相对于C449、C459和C459而言,菌株H2199、C598和C449缺乏自己的特异性抗原成分,因此,H2199- C449、C449- C459、C598 -C459之间的抗原关系可分别描述为 b- bc。基于上述结果,可将只与10型抗血清发生凝集反应的分离株分成4个亚型。

Fifty-eight Chinese isolates of Riemerella anatipestifer which agglutinated only in antiserum to serotype 10 representative strain (H2199) were further serotyped by agar gel precipitin methods. Based on precipitation patterns, these strains can be divided into four groups: the first group contained 30 isolates represented by C2, the second contained 3 isolates represented by C449, the third contained 6 isolates represented by C459, and the fourth contained 19 isolates represented by C598. Isolates in the first group showed identical to H2199, and isolates in other groups showed identical to representative strain of their own respectively, but had antigenic differences with H2199 or one another.Antigenic relationships between H2199 and four groups were further studied using slide and tube agglutination tests with adsorbed antiserum. Isolates represented by C2 were real serotype 10. Isolates identical to C459 showed distinct from H2199, such strains possessed their own specific antigenic materials,in addition they had antigens in common with H2199. The relationship between H2199 and C459 could be described as ab-bc. Similar antigenic relationships were observed between C598 and H2199, C598 and C449 respectively. However, compared with C449, C459 and C459 strains, H2199, C598 and C449 had no type-specific antigenic materials of their own in tube agglutination test respectively. So the relationship between H2199 and C449, C598 and C459, C449 and C459 can be described as b-bc respectively. It might be better to divide the isolates agglutinated in anti H2199 serum into subtypes 1～4 represented by strains H2199 (or C2), C449, C459, C598 respectively.