摘要
Random Amplified Polymorphic DNA (RAPD) analysis was used with 10 primers to assess genetic variability in Shanxi native goats (Yangcheng White,Licheng Daqing,Lüliang Black). The results showed as follows: 1.The DNA of 3 breeds provided a great number of molecular markers with high polymorphism. 2. According to their RAPD fingerprinting map, the genetic distance and average band similarity among 3 breeds were calculated, 0.182 7 between Yangcheng White Goat and Licheng Daqing Goat is the smallest. Cluster analysis also showed that Licheng Daqing Goat and Yangcheng White Goat from alike region had a smaller distance. 3. The genetic diversity and its partition within and between populations of the 3 populations were calculated by using Shannon index. The genetic diversity index of Licheng Daqing,Yangcheng White,Lüliang Black were 0.328 8,0.349 9 and 0.154 5 respectively, Genetic differentiation (Gst) was estimated to be about 68.49 among 3 populations. The results indicated that the genetic variation was mainly among breeds; the genetic character of Lüliang Black Goat was peculiar in native breeds indicating that protection and further selection should be strengthened.
Random Amplified Polymorphic DNA (RAPD) analysis was used with 10 primers to assess genetic variability in Shanxi native goats (Yangcheng White,Licheng Daqing,Lüliang Black). The results showed as follows: 1.The DNA of 3 breeds provided a great number of molecular markers with high polymorphism. 2. According to their RAPD fingerprinting map, the genetic distance and average band similarity among 3 breeds were calculated, 0.182 7 between Yangcheng White Goat and Licheng Daqing Goat is the smallest. Cluster analysis also showed that Licheng Daqing Goat and Yangcheng White Goat from alike region had a smaller distance. 3. The genetic diversity and its partition within and between populations of the 3 populations were calculated by using Shannon index. The genetic diversity index of Licheng Daqing,Yangcheng White,Lüliang Black were 0.328 8,0.349 9 and 0.154 5 respectively, Genetic differentiation (Gst) was estimated to be about 68.49 among 3 populations. The results indicated that the genetic variation was mainly among breeds; the genetic character of Lüliang Black Goat was peculiar in native breeds indicating that protection and further selection should be strengthened.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2005年第2期202-204,共3页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
山西省青年基金(20001041)
