PCR生物芯片/微装置在微生物检测中应用研究

Research on applications for PCR biochip/microdevice in detecting microbes

聚合酶链式反应(PCR)技术已经在分子生物学、生物化学、临床医学、遗传以及法医等领域得到广泛的应用。基于微电子机械系统技术开发而成的PCR生物芯片由于具有所需样品和反应混合物体积小、反应时间短、轻便等优点而日益受到人们的重视。介绍PCR生物芯片/微装置(包括反应池内固定扩增式和连续流动式)在微生物埃希氏大肠杆菌(E.coli)和微生物战剂检测中的应用。

The polymerase chain(PCR) reaction technique has been widely used in such fields as molecular biology,biochemistry,clinical medicine,genetics and forensic medicine.PCR biochip/microdevice developed on base of microelectromechnical systems (MEMS) is increasingly of great interest as a result of small volume of sample and reaction mixture,short reaction period and portability.The applications of PCR biochip/microdevice including the micro-chamber stationary PCR and the continuous-flow PCR are specially introduced in the fields of the detection for microbes-E.coli and war agents.