女性外阴尖锐湿疣及湿疣样病变组织中人类乳头瘤病毒 DNA 的检测

The Detection of DNA Type of Human Papilloma Virus in Condylomata Acuminata and Condylomalike Vulvar Lesions

用生物素和^(32)P-人类乳头瘤病毒 HPV11、HPV16和 HPV18型 DNA 探针,以斑点和 SouthernBlot 杂交方法检测了49例北京地区女性外阴尖锐湿疣和湿疣样病变组织中 HPV DNA。在24例尖锐湿疣组织中,HPV DNA 阳性者为18例(75%);其中 HPV11 DNA 阳性者16例,占阳性例数的88.9%;HPV16 DNA 阳性者2例,占阳性例数的11.1%;没有发现 HPV18 DNA 阳性者.25例湿疣样病变组织中,8例含有 HPV DNA(32%),全为 HPV11型。本文对湿疣样病变与 HPV 感染的关系,外阴部尖锐湿疣的分型及诊断标准进行了初步探讨。

40 cases(24 condylomata acuminata,25 condylomalike vulvar lesions)are examined for HPV DNA type by Dot and Southern Blot hybridization.75% of condylomata acuminata is positive,and 88.9% of positive cases is HPV11 type while 11.1% is of HPV 16 type.Com- paring with condylomata acuminata,only 32% of 25 condylomalike vulvar lesions contains HPV type 11 DNA.The classification,diagnostic criteria of condylomata acuminata as well as eitiology of condylomalike vulvar lesions are discussed.