对流免疫电泳、一维与二维电泳免疫印迹检测ENA抗体

A Study of ENA Antibodies with Counterimmuno-electrophoresis(CIE),One and Two Dimensional Electrophoresis and Immunoblotting(1D-IB,2D-IB)

用小牛胸腺、人脾脏、HEP-2细胞为抗原作对流免疫电泳,用 HEP-2细胞为抗原作一维(1D-IB)与二维电泳免疫印迹(2D-IB)。检测20例血清中的 ENA 抗体。ID-IB 唯独对检测 Sm 抗体比 CIE 敏感,2D-IB 可有效地检出 SS-A、SS-B 抗体并精细地分析其特异性,也能检出68、20kD 的 RNP 抗体,但在20例 SLE 血清中仅检测出3例 Sm 抗体。以 HEP-2细胞为抗原作 CIE,SS-B 抗体的检出很低(2/20).

Twenty SLE sera were examined for ENA autoantibodies with calf thymus and human spleen extracts,and HEP-2 cell lysate as antigens in CIE,and HEP-2 lysate as antigen in 1D-IB and 2D-IB.1D-IB was more sensitive than CIE for the detection of Sm antibody only. 2D-IB could effectively detect SS-A and SS-B antibodies and analyze their fine specificities; it could demonstrate RNP antibodies reacting with 68 kD and 20 kD polypeptides,but had a low detection rate for Sm antibody(3 positives out of 20 sera).The use of HEP-2 cell lysate as antigen in CIE yielded a low detection rate for SS-B antibody(2/20).