摘要
应用注入法制备了位径为250±21nm的左旋咪唑大单层脂质体,并获得20％以下的药物包裹率.包裹率受药脂比和脂质组成的影响.制备脂质体的有机相、脂质成分是影响左旋咪唑脂质体稳定性的重要因素.综合结果表明,以氯仿为有机相,药脂比定为1:100(W/W),脂质成分比为卵磷脂:胆固醇=5:1时,用100℃煮沸灭菌药物渗漏小于10％,且贮存稳定,是制备左旋咪唑脂质体的适宜条件.灭菌后,脂质体的形态经电镜观察无明显变化.
In this paper, preparation of Levamisole-liposomes was reported. The factorsaffecting the encapsulation ratio of LMS-liposomes and their stability were studied. A fast andeasy method for determining LMS-liposomes was established. The results suggested that LMS-liposomes with 20% encapsulation ratio can be obtained. The important factors affecting LMS-liposomes stability were cholesterol content in liposomes and the ratio of drug to liposome, etc.Ten percent of entrapped LMS was not retained after sterilization at 100℃ for 30 min.
