摘要
应用双抗体夹心ELISA对长春、内蒙等地区的244只绵羊进行了牛病毒性腹泻-粘膜病病毒(BVD—MDV)感染调查.结果,BVD—MDV的阳性感染率为14.6％～83.3％.应用电镜对部分ELISA阳性和阴性样品进行观察,结果7份阳性样品中均见有BVD—MDV粒子,5份阴性样品均未观察到BVD—MDV粒子.定期采取11只自然感染BVD—MD病毒的绵羊粪便进行检查,结果自然感染BVD—MDV的绵羊,排毒持续时间至少为3个月.本试验表明,羊感染BVD—MDV可长时间排毒,是潜在的危险传染源.
A sandwich ELISA was employed to detect the Bovine Viral Diarrhea-Mucosal Disease Virus (BVD-MDV) in feces of 244 sheep from Changchun and InnerMongolia area. The result indicated that the carrier rate of BVD-MDV in sheep variedfrom 14.6% to 83.3%. Examination of some fecal samples by electron microscopy hadconfirmed that BVD-MDV particles were found in ELISA-positive samples, whereas noBVD-MDV particles in ELISA-negative ones. Virus-shedding from those positive sheeplasted at least 3 months. The authors concluded that the sheep can carry and shed BVD-MDV for a long period of time and that they are a dangerous potential infection source.
