紫花苜蓿愈伤组织及体细胞胚的细胞学观察

Cytological observation on the callus and the somatic embryos arising of Medicago sativa

以紫花苜蓿Medicago sativa 的下胚轴为材料,诱导愈伤组织和体细胞胚的发生。在此过程中采用石蜡切片法观察愈伤组织和体细胞胚的内部结构,结果表明,胚性愈伤和非胚性愈伤在形态和细胞学上均有很大差别,体细胞胚的发生经历了多细胞原胚、球形胚、心形胚、子叶胚、鱼雷胚等多个阶段。观察发现,在胚性愈伤的内部和表面都有体细胞胚发生。

During inducing the callus of hypocotyls of Medicago sativa, histological and cytological observations of the callus and embryos were performed. The result showed much difference between embryonic callus and non-embryonic callus. Somatic embryos developed through multicellar proembryo, globular embryo, heart-shape embryo, Cotyledon-shape embryo, torpedo embryo. Somatic embryos can derived from both inner callus and outer callus.