高效液相色谱法测定川东獐牙菜中多种成分的含量

HPLC Determination of main constituents in Swertia davidi Franch.

目的:建立测定川东獐牙菜中雏菊叶龙胆苷、苦龙胆酯苷、去甲基雏菊叶龙胆酮和雏菊叶龙胆酮含量的高效液相色谱方法。方法:HYPERSIL BDS C_(18)(4.6mm×200mm,5μm)色谱柱,流动相为乙腈-磷酸二氢钠(0.03mol·L^(-1),pH=3.0),梯度洗脱(CH_3CN%:0 min 10%,15min 20%,30min 35%,50min 55%),流速为1.0mL·min^(-1),检测波长为250nm,柱温为25℃。结果:雏菊叶龙胆苷,苦龙胆酯苷,去甲基雏菊叶龙胆酮和雏菊叶龙胆酮分别在0.0403-0.3024μg,0.0604-0.4527μg,0.1320-0.9900μg,0.1610-1.2072μg范围内线性良好,相关系数r分别为0.9999,0.9999,0.9999,0.9999,其平均回收率均在98%-103%范围内,RSD均小于1%(n=5)。结论:本方法操作简便,分离效果好,灵敏度高,重现性好,为全面控制川东獐牙菜质量提供一个可靠的方法。

Objective: To established a method for the determination of swertianolin, amarogentin, demethylbellidifolin and bellidifolin in Swertia davidi Franch. Methods: The HYPERSIL BDS C_(18) (4. 6mm×200 mm,5μm)was used. The mobile phase was consisted of CH_3CN-NaH_2PO_4 (0.03mol·L^(-1) , pH=3.0) , gradient elution (CH_3CN%:0min 10% ,15 min 20% ,30 min 35% ,50 min 55%) ,The flow rate was 1.0 mL·min^(-1). The UV detection wavelength was 250 nm. Results: The calibration curves of swertianolin,amarogentin,demethylbellidifolin and bellidifolin were in good linearity over the range of 0.0403-0.3024μg,0.0604-0.4527μg,0.132-0.990μg, 0.1610-1.2072μg, respectively (r=0.9999,0.9999,0.9999,0.9999), The average recoveries were in the range of 98% and 103% , which RSD were less than 1% (n=5). Conclusion: The method is simple, sensitive, accurate and reproducible and can be used to control the quality of Swertia davidi Franch.