HPLC-ELSD测定桑叶中1-脱氧野尻霉素的含量

HPLC-ELSD Determination of 1-Deoxynojirimycin in Mulberry Leaves

目的:采用高效液相色谱法测定了桑叶中1-脱氧野尻霉素的含量。方法:应用EISD,SUPELCOSIL LC-NH_2(25cm×4.6mm,5μm)色谱柱,乙腈-水(80∶20)为流动相,流速0.8mL·min^(-1)。结果:1-脱氧野尻霉素在2.28-13.68μg范围内呈线性,平均回收率为96.1%(n=6),RSD为2.2%。结论:本法简便,快捷,结果可靠,可用于桑叶中生物碱的质量控制。

Objective:To develop a method to determine the contents of 1-deoxynojirimycin(1-DNJ) in Morus alba leaves. Method: With the use of Evaporative Light Scattering Detector(ELSD), an SUPELCOSIL LC-NH_2 (25 cm×4. 6 mm,5 μm) column and acetonitrile-water(80: 20) as mobile phase, the flow rate was 0. 8 mL·min^(-1). Results: The calibration curves of 1-deoxynojirimycin showed good linearity in the range of 2. 28-13. 68 μg,the average recoveries was 96. 1% (n= 6),with RSD of 2. 2%. Conclusion: It was proved that the method with HPLC-ELSD was simple, the results were reliable and accurate, it can be used for the quality control of Alkaloids in Morus alba leaves.