高效液相色谱法分离测定雪莲细胞中的黄酮

Separation and Determination of Flavonoids in Tissue Cultural Media of Medusa Saussurea(Saussurea Medusa) Cells by HPLC

目的:对水母雪莲细胞中的黄酮进行分析,为雪莲细胞培养条件及采收时间提供依据。方法:采用ZORBAX SB-C_(18)色谱柱(4.6mm×250mm,5μm),流动相:甲醇-水(50:50,v/v),磷酸调节pH≈4;流动相流速:0.7mL·min^(-1);检测波长:365nm。结果:雪莲细胞中检出了芦丁45.5,槲皮素29.0,木犀草素58.1和芹菜素9.6(单位:μg/g),线性范围:0.1-50μg·mL^(-1),回收率在85.8%-101.2%之间,相对标准偏差<7%(n=5)。结论:此方法分析雪莲细胞中的黄酮效果良好,可作为培养雪莲细胞的质量监控方法。

Objective: Flavonoids in Medusa Saussurea Cells were qualitative and quantitative analysis and it could be offer conditions of tissue culture of Medusa Saussurea. Method: Qualitative and quantitative analysis of flavonoids was carried out by HPLC with ZORBAX SB-C_(18)(4. 6mm×250mm,5μm)column,mobile phase consisted of CH_3OH-H_2O(50:50, v/v,adjust pH about 4 with phosphoric acid)at 0. 7mL·min^(-1) flow rate, wavelengh was 365nm. Results:Rutin,quercetin,luteolin and apigenin were determined in Medusa Saussurea Cells,the average recovery was 85. 8%-101. 2% with RSD less than 7%(n-5). Conclusion:The method was good for determination of flavonoids in Medusa Saussurea cells, it could be used for controlling guide line of tissue culture of Medusa Saussurea.