用Peterson法测定视明注射液中微量多肽的含量

Quantitative Determination of Microamount of Polypeptide in Shiming Injection by Peterson Method

目的:用Peterson法对视明注射液中多肽进行定量分析。方法:Peterson法。结果:视明注射液中的多肽标准曲线线性范围为:0.98—15.68μg(r=0.9997),回归方程:Y=0.00311+0.01516X,平均回收率为101.7%,RSD=2.3%(n=6)。结论:该方法简便,准确,回收率好,适于微量蛋白质或多肽的含量测定。

Objective:Determinate the polypeptide in Shiming injection by Peterson method. Method: Peterson method. Results:The standard curve for the polypeptide was linear over the range 0.98-15. 68μg(r=0.9997),typical equation:Y=0.00311+0.01516X, the average recovery was 101.7% , RSD=2. 3% (n=6). Conclusion:This method is simple, accurate with good recovery and is suitable for the quantitative determination of microgram quantities of protein (or polypeptide).