酒石酸锑钾诱导人结肠癌细胞凋亡

Potassium Antimonyl Tartrate Induces Apoptosis of Human Colon Cancer Cells

目的 研究表明酒石酸锑钾能抑制白血病细胞及淋巴瘤细胞生长,并诱导其凋亡。本研究旨在探讨酒石酸锑钾诱导人结肠癌SW480凋亡的作用。方法 将不同浓度酒石酸锑钾作用于体外培养人结肠癌SW480细胞。采用MTT法检测酒石酸锑钾对结肠癌SW480细胞的生长抑制作用,并计算生长抑制率;流式细胞术检测亚二倍体峰,并利用TUNEL法进行凋亡的分子生物学检测。结果 酒石酸锑钾能明显抑制结肠癌细胞生长,抑制作用呈时间和剂量依赖性;流式细胞仪检测到亚二倍体峰,亚二倍体细胞比例随时间延长而增加。TUNEL法观察到棕褐色着染的凋亡阳性细胞。结论 酒石酸锑钾能抑制SW480细胞生长并诱导细胞凋亡。

Objective This study was designed to investigate whether potassium antimonyl tartrate can induce apoptosis in human colon cancer cells (SGC-7901) in vitro,which provide experiment evidence for colon cancer therapy.Methods The growth inhibition of cells induced by various concentrations of PAT in different time course was analyzed by using MTT assay.The flow cytomery and terminal deoxynucleotidyl transferase-mediated duttp-biotin nick nedlabel (TNNEL) were applied to detect apoptotic cells.Results Potassium antimonyl tartrate inhibited SW480 cells growth significantly in does dependent and time dependent manner.DNA histograms showed the hypodiploid peak,the ration of hypodiploid cells was increased with the increase of dose and elongation of time.Bown-color positive apoptotic cells were observed with TUNEL method.Conclusion Potassium antimonyl tartrat can inhibit the proliferation of SW480 cells and induce apoptosis.